Ultrasensitive dual-quenching electrochemiluminescence immunosensor for prostate specific antigen detection based on graphitic carbon nitride quantum dots as an emitter

被引:7
作者
Liu, Pingkun [1 ]
Meng, Hui [1 ]
Zhang, Gui [1 ]
Song, Li [1 ]
Han, Qian [1 ]
Wang, Cun [1 ]
Fu, Yingzi [1 ]
机构
[1] Southwest Univ, Key Lab Luminescence Anal & Mol Sensing, Sch Chem & Chem Engn, Minist Educ, Chongqing 400715, Peoples R China
基金
中国国家自然科学基金;
关键词
Electrochemiluminescence; Dual-quenching effect; Gold nanoparticles modified manganese dioxide; Graphitic carbon nitride quantum dots; Silver nanoparticles doped multilayer Ti3C2 MXene hybrids; Immunosensor; RESONANCE ENERGY-TRANSFER; NANOTUBES; BIOSENSOR; MXENES; AU;
D O I
10.1007/s00604-021-05015-5
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Early monitoring of prostate-specific antigen (PSA) is crucial in diagnosis and proactive treatment of prostate disease. Herein, a dual-quenching ternary ECL immunosensor was designed for PSA detection based on graphitic carbon nitride quantum dots (g-CNQDs, as an emitter), potassium persulfate (K2S2O8, as a coreactant), and silver nanoparticles doped multilayer Ti3C2 MXene hybrids (Ag@TCM, as a coreaction accelerator). First, Ag@TCM was immobilized on the surface of a glassy carbon electrode, then g-CNQDs was further adsorbed on Ag@TCM to acquire a higher initial ECL signal at a potential window from - 1.3 to 0.0 V (vs. Ag/AgCl). Ag@TCM not only acted as the coreaction accelerator, but also as a matrix to load enormous g-CNQDs and prostate-specific capture antibody via Ag-N bond. Meanwhile, prostate-specific detection antibody was marked by gold nanoparticles modified manganese dioxide as a dual-quenching probe (Ab2- Au@MnO2). When Ab2-Au@MnO2 was introduced into the ternary ECL system via sandwiched immuno-reaction, the high-sensitive detection of PSA was achieved by the dual-quenching effect, caused by the resonant energy transfer from g-CNQDs (energy donor) to Au@MnO2 (energy acceptor). As a result, this ECL immunosensor showed a good dynamic concentration range from 10 fg.mL(-1) to 100 ng.mL(-1) with a detection limit of 6.9 fg.mL(-1) for PSA detection. The dual-quenching ECL strategy presented high stability and good specificity to open up a new pathway for ultrasensitive immunoassay.
引用
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页数:10
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