Light-cleavable rapamycin dimer as an optical trigger for protein dimerization

被引：43

作者：

Brown, Kalyn A. ^{[1
,2
]}

Zou, Yan ^{[2
]}

Shirvanyants, David ^{[3
]}

Zhang, Jie ^{[2
]}

Samanta, Subhas ^{[1
]}

Mantravadi, Pavan K. ^{[1
]}

Dokholyan, Nikolay V. ^{[3
]}

Deiters, Alexander ^{[1
,2
]}

机构：

[1] Univ Pittsburgh, Dept Chem, Pittsburgh, PA 15260 USA

[2] N Carolina State Univ, Dept Chem, Raleigh, NC 27695 USA

[3] Univ N Carolina, Dept Biochem & Biophys, Chapel Hill, NC 27599 USA

来源：

CHEMICAL COMMUNICATIONS | 2015年 / 51卷 / 26期

基金：

美国国家科学基金会;

关键词：

SITE-SPECIFIC RECOMBINATION; CRE RECOMBINASE; TEV PROTEASE; ESCHERICHIA-COLI; EUKARYOTIC CELLS; GENE-FUNCTION; ACTIVATION; EXPRESSION; POLYMERASE; INDUCTION;

D O I：

10.1039/c4cc09442e

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Rapamycin-induced protein heterodimerization of FKBP12 and FRB is one of the most commonly employed switches to conditionally control biological processes. We developed an optically activated rapamycin dimer that does not induce FKBP12-FRB dimerization until exposed to light, and applied it to control kinase, protease, and recombinase function.

引用

页码：5702 / 5705

页数：4

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