A recombinant pseudorabies virus co-expressing capsid proteins precursor P1-2A of FMDV and VP2 protein of porcine parvovirus: a trivalent vaccine candidate

被引：42

作者：

Hong, Qi ^{[1
]}

Qian, Ping ^{[1
]}

Li, Xiang-Min ^{[1
]}

Yu, Xiao-Lan ^{[1
]}

Chen, Huan-Chun ^{[1
]}

机构：

[1] Huazhong Agr Univ, Coll Vet Med Sci, Lab Anim Virol, Wuhan 430070, Peoples R China

来源：

BIOTECHNOLOGY LETTERS | 2007年 / 29卷 / 11期

关键词：

foot-and-mouth disease virus; porcine parvovirus; recombinant pseudorabies virus;

D O I：

10.1007/s10529-007-9459-6

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Pseudorabies (PR), foot-and-mouth disease (FMD), and porcine parvovirus disease are three important infectious diseases in swine worldwide. The gene-deleted pseudorabies virus (PRV) has been used as a live-viral vector to develop multivalent genetic engineering vaccine. In this study, a recombinant PRV, which could co-express protein precursor P1-2A of FMDV and VP2 protein of PPV, was constructed using PRV TK-/gE(-)/LacZ(+) mutant as the vector. After homologous recombination and plaque purification, recombinant virus PRV TK-/ gE(-)/P1-2A-VP2 was acquired and identified. Immunogenicity, safety of the recombinant PRV and its protection against PRV were confirmed in a mouse model by indirect ELISA and serum neutralization test. The results show that the recombinant PRV is a candidate vaccine strain to develop a novel trivalent vaccine against PRV, FMDV and PPV in swine.

引用

页码：1677 / 1683

页数：7

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