Simultaneous determination of vitamin C and its carbamylated derivatives by high-performance liquid chromatography with post-column derivatization

被引：12

作者：

Koshiishi, I ^{[1
]}

Mamura, Y ^{[1
]}

Imanari, T ^{[1
]}

机构：

[1] Chiba Univ, Fac Pharmaceut Sci, Chiba 263, Japan

来源：

JOURNAL OF CHROMATOGRAPHY A | 1998年 / 806卷 / 02期

关键词：

vitamins; ascorbate; dehydroascorbate; 2,3-diketogulonate; carbamyl ascorbate;

D O I：

10.1016/S0021-9673(98)00074-0

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A highly sensitive method for the simultaneous determination of ascorbate (AsA), dehydroascorbate (DHA), 2,3-diketogulonate (2,3-DKG), carbamyl ascorbate (CAA) and carbamylated dehydroascorbate derivative (CDA) was developed by high-performance liquid chromatography with post-column derivatization. The successful separation of these substances was achieved by an adsorption chromatography using poly(ethylene glycol) copolymer as a packing material in the separation column. For the detection, each substance was boiled with benzamidine in alkaline solution, producing fluorescence products. Both CAA and CDA were alkaline-labile, degrading to AsA and 2,3-DKG, so that these carbamylated derivatives could be detected in a similar manner as AsA and 2,3-DKG, respectively. The detection limits for quantitative determination of these substances were less than 0.5 mu M, and the coefficients of variation of the peak areas were in the range of 2.2 to 2.8%. The usefulness and practicability of the present method were verified by application to the determination of these substances in plant leaves soaked in 0.5 M sodium cyanate solution. (C) 1998 Elsevier Science B.V.

引用

页码：340 / 344

页数：5

共 14 条

[1] POTASSIUM CYANATE AS AN INHIBITOR OF SICKLING OF ERYTHROCYTES IN-VITRO (UREA/HEMOGLOBIN S/CARBAMYLATED VALINE) [J].