The invertor knock-in conditional chromosomal translocation mimic

被引：27

作者：

Forster, A ^{[1
]}

Pannell, R ^{[1
]}

Drynan, LF ^{[1
]}

Codrington, R ^{[1
]}

Daser, A ^{[1
]}

Metzler, M ^{[1
]}

Lobato, MN ^{[1
]}

Rabbitts, TH ^{[1
]}

机构：

[1] MRC, Mol Biol Lab, Cambridge CB2 2QH, England

来源：

NATURE METHODS | 2005年 / 2卷 / 01期

关键词：

D O I：

10.1038/NMETH727

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Knock-in models of tumor-specific chromosomal translocations can generate Lethal mutations. To circumvent this, a new conditional gene fusion model has been developed (invertor mice) and exemplified with the Ews-ERG fusion oncogene. An ERG segment, flanked by loxP sites, was knocked in to an intron of the Ews gene but in an inverted transcription orientation and lineage-specific Ews-ERG fusion created by Cre-mediated inversion. This invertor method is a completely conditional approach, applicable to any gene fusion, to emulate effects of translocations found in human cancers.

引用

页码：27 / 30

页数：4

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