The retinoid X receptor from mud crab: new insights into its roles in ovarian development and related signaling pathway

被引：36

作者：

Gong, Jie ^{[1
,2
]}

Huang, Chencui ^{[1
]}

Shu, Ling ^{[1
]}

Bao, Chenchang ^{[1
]}

Huang, Huiyang ^{[1
]}

Ye, Haihui ^{[1
,3
]}

Zeng, Chaoshu ^{[1
,4
]}

Li, Shaojing ^{[1
]}

机构：

[1] Xiamen Univ, Coll Ocean & Earth Sci, Xiamen 361102, Peoples R China

[2] Nantong Univ, Sch Life Sci, Nantong 226007, Peoples R China

[3] Fujian Collaborat Innovat Ctr Exploitat & Utiliza, Xiamen 361102, Peoples R China

[4] James Cook Univ, Coll Marine & Environm Sci, Townsville, Qld 4811, Australia

来源：

SCIENTIFIC REPORTS | 2016年 / 6卷

基金：

中国国家自然科学基金;

关键词：

METHYL FARNESOATE; ECDYSONE RECEPTOR; JUVENILE-HORMONE; FIDDLER-CRAB; ECDYSTEROID RECEPTOR; LIMB REGENERATION; MOLECULAR-CLONING; SCYLLA-PARAMAMOSAIN; EXPRESSION ANALYSIS; BINDING PROPERTIES;

D O I：

10.1038/srep23654

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

In arthropods, retinoid X receptor (RXR) is a highly conserved nuclear hormone receptor. By forming a heterodimeric complex with the ecdysone receptor (EcR), RXR is known to be vital importance for various physiological processes. However, in comparison to EcR, the RXR signaling pathway and its roles in crustacean reproduction are poorly understood. In the present study, the RXR mRNA was detected in the ovarian follicular cells of mud crab Scylla paramamosain (SpRXR) and during ovarian maturation, its expression level was found to increase significantly. In vitro experiment showed that both SpRXR and vitellogenin (SpVg) mRNA in the ovarian explants were significantly induced by 20-hydroxyecdysone (20E) but not methyl farnesoate (MF). However, differing from the in vitro experiment, injection of MF in in vivo experiment significantly stimulated the expressions of SpRXR and SpVg in female crabs at early vitellogenic stage, but the ecdysone and insect juvenile hormone (JH) signaling pathway genes were not induced. The results together suggest that both MF and SpRXR play significant roles in regulating the expression of SpVg and ovarian development of S. paramamosain through their own specific signaling pathway rather than sharing with the ecdysone or the insect JH.

引用

页数：13

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