BMP signaling in wing development: A critical perspective on quantitative image analysis

被引:8
作者
Brooks, Alexander [1 ]
Dou, Wei
Yang, Xiaoying
Brosnan, Tara [1 ]
Pargett, Michael [2 ]
Raftery, Laurel A. [1 ]
Umulis, David M. [2 ]
机构
[1] Univ Nevada, Sch Life Sci, Las Vegas, NV 89154 USA
[2] Purdue Univ, Weldon Sch Biomed Engn, W Lafayette, IN 47907 USA
关键词
Quantitative image analysis; Mathematical modeling; Wing development; Drosophila; Normalization; MORPHOGEN GRADIENT FORMATION; EARLY DROSOPHILA EMBRYO; STEM-CELL NICHE; GENE-EXPRESSION; FLUORESCENCE MICROSCOPY; POSITIONAL INFORMATION; IMAGINAL DISCS; DPP; PRECISION; DYNAMICS;
D O I
10.1016/j.febslet.2012.03.050
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Bone Morphogenetic Proteins (BMPs) are critical for pattern formation in many animals. In numerous tissues, BMPs become distributed in spatially non-uniform profiles. The gradients of signaling activity can be detected by a number of biological assays involving fluorescence microscopy. Quantitative analyses of BMP gradients are powerful tools to investigate the regulation of BMP signaling pathways during development. These approaches rely heavily on images as spatial representations of BMP activity levels, using them to infer signaling distributions that inform on regulatory mechanisms. In this perspective, we discuss current imaging assays and normalization methods used to quantify BMP activity profiles with a focus on the Drosophila wing primordium. We find that normalization tends to lower the number of samples required to establish statistical significance between profiles in controls and experiments, but the increased resolvability comes with a cost. Each normalization strategy makes implicit assumptions about the biology that impacts our interpretation of the data. We examine the tradeoffs for normalizing versus not normalizing, and discuss their impacts on experimental design and the interpretation of resultant data. (C) 2012 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
引用
收藏
页码:1942 / 1952
页数:11
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