An alkali stable cellulase by chemical modification using maleic anhydride

被引：13
|
作者：
Bund, RK [1 ]
Singhal, RS [1 ]
机构：
[1] Univ Bombay, Dept Chem Technol, Food & Fermentat Technol Div, Bombay 400019, Maharashtra, India
来源：
CARBOHYDRATE POLYMERS | 2002年 / 47卷 / 02期
关键词：
akali stable cellulase; chemical modification; maleic anhydride;
D O I：
10.1016/S0144-8617(01)00182-5
中图分类号：
O69 [应用化学];
学科分类号：
081704 ;
摘要：
An alkali stable cellulase was obtained by chemical modification with 0.5 M maleic anhydride at 2-4 degreesC and pH 8.0 for 25 min. The cellulase so modified showed a marginally better activity in alkaline range as compared to native cellulase. It had a half-life of 462.0, 433.13, 128.33 and 144.38 h vis-h-vis 13.08, 121.58, 126.0 and 187.30 for native unmodified enzyme at a pH of 8.0, 9.0, 10.0 and 11.0, respectively, at 50 degreesC. These results could have promising applications in textiles and detergents. (C) 2002 Elsevier Science Ltd. All rights reserved.
引用
收藏
页码：137 / 141
页数：5
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