An alkali stable cellulase by chemical modification using maleic anhydride

被引：13

作者：

Bund, RK ^{[1
]}

Singhal, RS ^{[1
]}

机构：

[1] Univ Bombay, Dept Chem Technol, Food & Fermentat Technol Div, Bombay 400019, Maharashtra, India

来源：

CARBOHYDRATE POLYMERS | 2002年 / 47卷 / 02期

关键词：

akali stable cellulase; chemical modification; maleic anhydride;

D O I：

10.1016/S0144-8617(01)00182-5

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

An alkali stable cellulase was obtained by chemical modification with 0.5 M maleic anhydride at 2-4 degreesC and pH 8.0 for 25 min. The cellulase so modified showed a marginally better activity in alkaline range as compared to native cellulase. It had a half-life of 462.0, 433.13, 128.33 and 144.38 h vis-h-vis 13.08, 121.58, 126.0 and 187.30 for native unmodified enzyme at a pH of 8.0, 9.0, 10.0 and 11.0, respectively, at 50 degreesC. These results could have promising applications in textiles and detergents. (C) 2002 Elsevier Science Ltd. All rights reserved.

引用

页码：137 / 141

页数：5

共 14 条

[1] Cellulose degrading enzymes and their potential industrial applications [J].

Bhat, MK ;

Bhat, S .

BIOTECHNOLOGY ADVANCES, 1997, 15 (3-4) :583-620

[2] MEASUREMENT OF CELLULASE ACTIVITIES [J].

GHOSE, TK .

PURE AND APPLIED CHEMISTRY, 1987, 59 (02) :257-268

[3] CHARACTERISTICS OF CELLULASE MODIFIED WITH A COPOLYMER OF POLYETHYLENE-GLYCOL DERIVATIVE AND MALEIC-ACID ANHYDRIDE [J].

KAJIUCHI, T ;

PARK, JW .

JOURNAL OF CHEMICAL ENGINEERING OF JAPAN, 1992, 25 (02) :202-206

[4] ADSORPTION CONTROL OF CELLULASE ONTO CELLULOSE BY MODIFICATION WITH AMPHIPHILIC COPOLYMER [J].

KAJIUCHI, T ;

PARK, JW ;

MOON, HY .

JOURNAL OF CHEMICAL ENGINEERING OF JAPAN, 1993, 26 (01) :28-33

[5]

MEANS GE, 1971, SELECTED TECHNIQUES, P215

[6] PROTEIN STABILIZATION VIA HYDROPHILIZATION - COVALENT MODIFICATION OF TRYPSIN AND ALPHA-CHYMOTRYPSIN [J].

MOZHAEV, VV ;

SIKSNIS, VA ;

MELIKNUBAROV, NS ;

GALKANTAITE, NZ ;

DENIS, GJ ;

BUTKUS, EP ;

ZASLAVSKY, BY ;

MESTECHKINA, NM ;

MARTINEK, K .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1988, 173 (01) :147-154

[7]

Murphy A, 1998, BIOTECHNOL BIOENG, V58, P366, DOI 10.1002/(SICI)1097-0290(19980520)58:4<366::AID-BIT3>3.0.CO

[8]

2-H

[9] STABILITY OF ALANINE AMINOTRANSFERASE IS ENHANCED BY CHEMICAL MODIFICATION [J].

OFAGAIN, C ;

OKENNEDY, R ;

KILTY, C .

ENZYME AND MICROBIAL TECHNOLOGY, 1991, 13 (03) :234-239

[10] DEVELOPMENT OF EFFECTIVE MODIFIED CELLULASE FOR CELLULOSE HYDROLYSIS PROCESS [J].

PARK, JW ;

KAJIUCHI, T .

BIOTECHNOLOGY AND BIOENGINEERING, 1995, 45 (04) :366-373

← 1 2 →