Exploring the antioxidant potential of Teucrium polium extracts by HPLC-SPE-NMR and on-line radical-scavenging activity detection

被引：31

作者：

Goulas, Vlassios ^{[2
]}

Maria Gomez-Caravaca, Ana ^{[3
]}

Exarchou, Vassiliki ^{[1
]}

Gerothanassis, Ioannis R. ^{[2
]}

Segura-Carretero, Antonio ^{[3
]}

Fernandez Gutierrez, Alberto ^{[3
]}

机构：

[1] Univ Ioannina, NMR Ctr, GR-45110 Ioannina, Greece

[2] Univ Ioannina, Sect Organ Chem & Biochem, Dept Chem, GR-45110 Ioannina, Greece

[3] Univ Granada, Dept Analyt Chem, Fac Sci, E-18071 Granada, Spain

来源：

LWT-FOOD SCIENCE AND TECHNOLOGY | 2012年 / 46卷 / 01期

关键词：

Flavonoids; Plant extracts; HPLC-SPE-NMR; HPLC-DPPH; Phenylpropanoid glycosides; Radical-scavenging activity; LEMON VERBENA; IDENTIFICATION; FLAVONOIDS; GLYCOSIDE; LABIATAE;

D O I：

10.1016/j.lwt.2011.10.019

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

Teucrium polium is a popular medicinal plant that is used in the daily diet and the in vitro and in vivo antioxidant potency of its extracts has been widely investigated. In the present work, polar extracts from T polium were analyzed in terms of its composition and radical-scavenging activity with the employment of the state-of-the-art HPLC-SPE-NMR and HPLC-DPPH techniques in the search of new antioxidant agents for food industry. NMR and MS data revealed the presence of phenylpropanoid glycosides verbascoside and poliumoside, the flavones apigenin and its derivatives and two methoxyflavones. The on-line DPPH experiments showed that poliumoside is the most active component of the extracts and the antioxidant potential of T. polium polar extracts is mainly attributed to phenylpropanoid glycosides (66-80%). Our results suggested that T. polium extracts could be a promising source of natural antioxidants. This holistic approach also revealed that poliumoside or polar extracts of T. polium can be used in food industry as antioxidant agents with natural origin. The HPLC-SPE-NMR and HPLC-DPPH analysis of the extracts was performed for first time in the same device without any modification of the instrumentation, avoiding the need for isolation of the individual components. (C) 2011 Elsevier Ltd. All rights reserved.

引用

页码：104 / 109

页数：6

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