Oxidant stress in nephrotic syndrome:: comparison of F2-isprostanes and plasma antioxidant potential

被引:36
作者
Dogra, G
Ward, N
Croft, KD
Mori, TA
Barrett, PHR
Herrmann, SE
Irish, AB
Watts, GF
机构
[1] Univ Western Australia, Dept Med, Perth, WA 6009, Australia
[2] Univ Western Australia, Western Australian Heart Res Inst, Perth, WA 6009, Australia
[3] Royal Perth Hosp, Dept Nephrol, Perth, WA, Australia
基金
英国医学研究理事会;
关键词
anti-oxidant defence; dyslipidaemia; F-2-isoprostanes; nephrotic syndrome; oxidant stress; oxygen radical absorbance capacity (ORAC);
D O I
10.1093/ndt/16.8.1626
中图分类号
R3 [基础医学]; R4 [临床医学];
学科分类号
1001 ; 1002 ; 100602 ;
摘要
Background. The nephrotic syndrome (NS) is associated with an increased risk of coronary heart disease. Increased oxidant stress may contribute to this by means of hyperlipidaemia and/or hypoalbuminaemia. In this study we assessed the contributory role of oxidant stress, as measured by F-2-isoprostanes and plasma oxygen radical absorbance capacity (ORAC), in subjects with NS. Methods. We studied 14 subjects with NS and 17 age- and sex-matched healthy non-proteinuric controls. Measurement of plasma and urinary F-2-isoprostanes was carried out using a combination of silica and reverse-phase cartridges, high-performance liquid chromatography, and gas chromatography mass spectrometry using electron-capture negative ionization. The plasma ORAC assay measured the decrease in fluorescence of phycoerythrin added to plasma in the presence of a free-radical generator. The ORAC value (muM) was calculated as the ratio of the area under the fluorescence decay curve for plasma to the area under the fluorescence decay curve for a Trolox standard. Results. Plasma ORAC was significantly lower in NS patients compared with controls: mean (standard error) NS patients 3306 muM (286); controls 4882 muM (496), P=0.011. In univariate linear regression analysis, plasma albumin was significantly positively correlated with plasma ORAC (r=0.40, P=0.03). Plasma and urinary F-2-isoprostanes did not differ significantly between NS and control groups. Conclusions. This study demonstrates that in the NS there is decreased free-radical trapping capacity of plasma that is inversely correlated with hypoalbuminaemia, but no increase in plasma and urinary F-2-isoprostanes. Decreased total plasma antioxidant potential in combination with hyperlipidaemia may contribute to the increased risk of cardiovascular disease seen in NS.
引用
收藏
页码:1626 / 1630
页数:5
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