Transplantation of marrow cells from children with standard risk-acute lymphoblastic leukemia at the end of therapy into NOD/SCID mice for detecting residual leukemic cells with in vivo growth potential

被引：7

作者：

Ramírez, M ^{[1
]}

Díaz, MA

Madero, L

Bueren, JA

机构：

[1] Hosp Nino Jesus, Unidad Invest, Madrid, Spain

[2] CIEMAT, Unidad Hematopoyesis & Terapia Gen, E-28040 Madrid, Spain

[3] Univ Autonoma Madrid, Hosp Nino Jesus, Unidad Oncol Pediat, Madrid, Spain

来源：

LEUKEMIA RESEARCH | 2003年 / 27卷 / 12期

关键词：

acute lymphoblastic leukemia; children; residual disease; NOD/SCID mice;

D O I：

10.1016/S0145-2126(03)00119-X

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

In the present work, we developed a strategy for detecting residual leukemia in the marrow of children with standard risk-acute lymphoblastic leukemia (sr-ALL) at the end of therapy, based on the capacity of human leukemic cells for growing in the NOD/SCID mice marrow microenvironment. Mononuclear (MN) marrow cells from 62 patients were injected into sublethally irradiated NOD/SCID mice and the engraftment kinetics and composition of the human grafts were determined periodically. The presence of human leukemic cells with immunophenotypes and clonal DNA markers similar to those of the original leukemic clone was studied. (C) 2003 Elsevier Ltd. All rights reserved.

引用

页码：1153 / 1157

页数：5

共 20 条

[1] NOD/SCID mice transplanted with marrow from patients with myelodysplastic syndrome (MDS) show long-term propagation of normal but not clonal human precursors [J].