Low- to high-throughput analysis of telomerase modulators with Telospot

被引：28

作者：

Cristofari, Gael

Reichenbach, Patrick

Regamey, Pierre-Olivier

Banfi, Damiano

Chambon, Marc

Turcatti, Gerardo

Lingner, Joachim

机构：

[1] Swiss Inst Expt Canc Res, CH-1066 Epalinges, Switzerland

[2] Ecole Polytech Fed Lausanne, Sch Life Sci, CH-1015 Lausanne, Switzerland

[3] Frontiers Genet Natl Ctr Competence Res, CH-1211 Geneva, Switzerland

[4] Ecole Polytech Fed Lausanne, Biomol Screening Facil, CH-1015 Lausanne, Switzerland

来源：

NATURE METHODS | 2007年 / 4卷 / 10期

关键词：

D O I：

10.1038/NMETH1099

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We designed a method termed Telospot to discover and characterize telomerase modulators as anticancer drugs or chemical biology tools. Telospot is based on a highly efficient human telomerase expression system and the detection of telomerase DNA reaction products in macroarray format. Telospot offers a highly scalable, cost- and time-effective alternative to presently available telomerase assays, which are limited by the requirement for PCR, telomerase purification or technologies not amenable to high throughput.

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页码：851 / 853

页数：3

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