Extraction, isolation, characterisation, antioxidant and anti-fatigue activities of Pleurotus eryngii polysaccharides

被引：24
|
作者：
Zhao, Yuanyuan [1 ]
Chen, Xuefeng [1 ]
Jia, Wei [1 ]
Gong, Guoli [1 ]
Zhao, Yanni [1 ]
Li, Guoliang [1 ]
Zhou, Jie [2 ]
Li, Xiaona [2 ]
Zhao, Yu [2 ]
Ma, Wenjin [3 ]
机构：
[1] Shaanxi Univ Sci & Technol, Sch Food & Biol Engn, Xian 710021, Peoples R China
[2] Xian Med Univ, Xian 710021, Peoples R China
[3] Chinese Acad Sci, Lanzhou Inst Chem Phys, Lanzhou 730000, Peoples R China
来源：
INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY | 2020年 / 55卷 / 06期
基金：
中国国家自然科学基金;
关键词：
Anti-fatigue activity; antioxidant activity; ethanol precipitation; hot water extraction; Pleurotus eryngii polysaccharides; structural features; STRUCTURAL-CHARACTERIZATION; PHYSICOCHEMICAL PROPERTIES; PANAX-GINSENG; IN-VITRO; PURIFICATION; WATER; PROLIFERATION; FRACTIONATION; EXERCISE; FEATURES;
D O I：
10.1111/ijfs.14501
中图分类号：
TS2 [食品工业];
学科分类号：
0832 ;
摘要：
Pleurotus eryngii polysaccharide (PEP) was obtained using hot water extraction and ethanol precipitation. Three purified polysaccharide fractions (namely PEP1-A, PEP2-A and PEP3-A) were obtained from PEP using DEAE-cellulose-52 chromatography and a gel permeation Sephadex G-100 column. Firstly, this paper examined the characterisation of PEP1-A, PEP2-A and PEP3-A. The corresponding molecular weights were 5.378 x 10(5), 9.506 x 10(6) and 4.975 x 10(5) Da, respectively. PEP1-A, PEP2-A and PEP3-A had similar monosaccharide compositions. PEP1-A was beta-configuration, and PEP2-A and PEP3-A were alpha-configuration. PEP1-A, PEP2-A and PEP3-A had pyran-type rings, (1 -> 3) glucose and (1 -> 6) galactose linkages. Secondly, PEP, PEP1-A, PEP2-A and PEP3-A possessed antioxidant activities, and PEP was best. Therefore, only PEP was used to study its anti-fatigue activity in vivo. The result proved that PEP had anti-fatigue activity. PEP could be used as a valuable natural food supplement for preventing anti-fatigue or functional food.
引用
收藏
页码：2492 / 2508
页数：17
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