Storage of Yarrowia lipolytica lipase after spray-drying in the presence of additives

被引：28
作者：
Alloue, Waze Aime Mireille
Destain, Jacqueline
Amighi, Karim
Thonart, Philippe
机构：
[1] Fac univ Sci Agron Gembloux, CWBI, Unite Bioind, B-5030 Gembloux, Belgium
[2] Univ Libre Bruxelles, Pharm Galen & Biopharm Lab, B-1050 Brussels, Belgium
来源：
PROCESS BIOCHEMISTRY | 2007年 / 42卷 / 09期
关键词：
Yarrowia lipolytica lipase; formulation; spray-drying; storage;
D O I：
10.1016/j.procbio.2007.05.024
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Lipase from Yarrowia lipolytica is an enzyme that presents numerous potentialities for biotechnological applications. This work describes the development of powders obtained by atomization of supernatants lipase from Y lipolytica LGx6481. Two formulations were studied: one formulation with skim milk powder and gum arabic, and the other with maltodextrin, calcium chloride and gum arabic. After drying, powders were stored at 4 and 20 degrees C in aluminium hermetic bags to evaluate their stability over a period of one year. The influence of water activity and glass transition temperature (T.) on the powder's storage were also studied. (c) 2007 Elsevier Ltd. All rights reserved.
引用
收藏
页码：1357 / 1361
页数：5
相关论文
共 27 条
[1]   Bacterial lipolytic enzymes: classification and properties [J].
Arpigny, JL ;
Jaeger, KE .
BIOCHEMICAL JOURNAL, 1999, 343 :177-183
[2]  
Bare G, 1999, APPL BIOCHEM BIOTECH, V77-9, P857
[3]  
BEAL C, 2003, Patent No. 2829147
[4]   Stabilization of Penicillium occitanis cellulases by spray drying in presence of Maltodextrin [J].
Belghith, H ;
Chaabouni, SE ;
Gargouri, A .
ENZYME AND MICROBIAL TECHNOLOGY, 2001, 28 (2-3) :253-258
[5]  
BROCKERHOFF H, 1971, J BIOL CHEM, V246, P5828
[6]   On the molecular characteristics, compositional properties, and structural-functional mechanisms of maltodextrins: A review [J].
Chronakis, IS .
CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION, 1998, 38 (07) :599-637
[7]   Improvement of lipase production from Yarrowia lipolytica [J].
Destain, J ;
Roblain, D ;
Thonart, P .
BIOTECHNOLOGY LETTERS, 1997, 19 (02) :105-107
[8]  
DESTIAN J, 1998, THESIS U SCI AGRONOM, P211
[9]   Molecular characteristics of maltodextrins and rheological behaviour of diluted and concentrated solutions [J].
Dokic, P ;
Jakovevic, J ;
Dokic-Baucal, L .
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS, 1998, 141 (03) :435-440
[10]   Production and down-stream processing of an extracellular lipase from the yeast Yarrowia lipolytica [J].
Fickers, P ;
Ongena, M ;
Destain, J ;
Weekers, F ;
Thonart, P .
ENZYME AND MICROBIAL TECHNOLOGY, 2006, 38 (06) :756-759
123