Storage of Yarrowia lipolytica lipase after spray-drying in the presence of additives

被引：28

作者：

Alloue, Waze Aime Mireille

Destain, Jacqueline

Amighi, Karim

Thonart, Philippe

机构：

[1] Fac univ Sci Agron Gembloux, CWBI, Unite Bioind, B-5030 Gembloux, Belgium

[2] Univ Libre Bruxelles, Pharm Galen & Biopharm Lab, B-1050 Brussels, Belgium

来源：

PROCESS BIOCHEMISTRY | 2007年 / 42卷 / 09期

关键词：

Yarrowia lipolytica lipase; formulation; spray-drying; storage;

D O I：

10.1016/j.procbio.2007.05.024

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Lipase from Yarrowia lipolytica is an enzyme that presents numerous potentialities for biotechnological applications. This work describes the development of powders obtained by atomization of supernatants lipase from Y lipolytica LGx6481. Two formulations were studied: one formulation with skim milk powder and gum arabic, and the other with maltodextrin, calcium chloride and gum arabic. After drying, powders were stored at 4 and 20 degrees C in aluminium hermetic bags to evaluate their stability over a period of one year. The influence of water activity and glass transition temperature (T.) on the powder's storage were also studied. (c) 2007 Elsevier Ltd. All rights reserved.

引用

页码：1357 / 1361

页数：5

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