Modulation of α-tropomyosin expression by α-tocopherol in rat vascular smooth muscle cells

被引：66
作者：
Aratri, E [1 ]
Spycher, SE [1 ]
Breyer, I [1 ]
Azzi, A [1 ]
机构：
[1] Univ Bern, Inst Biochem & Mol Biol, CH-3012 Bern, Switzerland
来源：
FEBS LETTERS | 1999年 / 447卷 / 01期
关键词：
alpha-tropomyosin; alpha-tocopherol; beta-tocopherol; vitamin E; differential display; smooth muscle cell;
D O I：
10.1016/S0014-5793(99)00277-X
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The effect of alpha-tocopherol (vitamin E) on gene expression in rat vascular smooth muscle cells mas studied by the differential display technique. One gene out of about 1000 genes analyzed, identified as alpha-tropomyosin, showed an increased transcription level caused by cr-tocopherol treatment. Northern and Western blot analysis revealed a time-dependent transient up-regulation of the amount of mRNA (peak between 2 and 3 h) and protein (peak at 5 h) in alpha-tocopherol-treated cells. No effect mas observed in cells treated with beta-tocopherol. (C) 1999 Federation of European Biochemical Societies.
引用
收藏
页码：91 / 94
页数：4
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