An atypical component of RNA-directed DNA methylation machinery has both DNA methylation-dependent and -independent roles in locus-specific transcriptional gene silencing

被引：31

作者：

Liu, Jun ^{[1
]}

Bai, Ge ^{[2
,3
]}

Zhang, Cuijun ^{[1
]}

Chen, Wei ^{[2
]}

Zhou, Jinxing ^{[1
]}

Zhang, Suwei ^{[1
]}

Chen, Qing ^{[1
]}

Deng, Xin ^{[2
,4
]}

He, Xin-Jian ^{[1
]}

Zhu, Jian-Kang ^{[2
]}

机构：

[1] Natl Inst Biol Sci, Beijing 102206, Peoples R China

[2] Purdue Univ, Dept Hort & Landscape Architecture, W Lafayette, IN 47907 USA

[3] Shanghai Jiao Tong Univ, Sch Agr & Biol, Shanghai 200240, Peoples R China

[4] Chinese Acad Sci, Inst Bot, Beijing 100093, Peoples R China

来源：

CELL RESEARCH | 2011年 / 21卷 / 12期

基金：

美国国家卫生研究院;

关键词：

DTF1; siRNA; DNA methylation; transcriptional gene silencing; RdDM; ARABIDOPSIS-THALIANA; GLYCOSYLASE/LYASE ROS1; PROCESSING CENTER; POLYMERASES-IV; SIRNA PATHWAY; PROTEIN; DEMETHYLATION; HETEROCHROMATIN; GENOME; PLANTS;

D O I：

10.1038/cr.2011.173

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

RNA-directed DNA methylation (RdDM) is an important de novo DNA methylation pathway in plants. RdDM mediates the transcriptional silencing of many endogenous genomic loci, most of which are transposon related. A forward genetics screen identified DTF1 (DNA-binding transcription factor 1) as a new component for RdDM in Arabidopsis. Loss-of-function mutations in DTF1 release the transcriptional silencing of RdDM target loci and reduce the accumulation of 24-nt small interfering RNAs (siRNAs) from some of the targets. Interestingly, in the dtf1 mutant plants, the release of transcriptional gene silencing at solo-LTR is accompanied by decreased siRNA accumulation but not by reduced DNA methylation. These results suggest that DTF1 is an atypical component of RdDM and has both DNA methylation-dependent and -independent roles in transcriptional gene silencing. We suggest that besides DNA methylation, siRNAs may cause some other uncharacterized epigenetic modifications that lead to transcriptional gene silencing.

引用

页码：1691 / 1700

页数：10

共 54 条

[1] Role of the Arabidopsis DNA glycosylase/lyase ROS1 in active DNA demethylation [J].