Impact of immunotherapy on blood dendritic cells in patients with Hymenoptera venom allergy

被引：30

作者：

Dreschler, Katharina ^{[1
]}

Bratke, Kai ^{[1
]}

Petermann, Sebastian ^{[1
]}

Bier, Andrea ^{[1
]}

Thamm, Petra ^{[1
]}

Kuepper, Michael ^{[1
]}

Virchow, J. Christian ^{[1
]}

Lommatzsch, Marek ^{[1
]}

机构：

[1] Univ Rostock, Zentrum Innere Med, Abt Pneumol, D-18057 Rostock, Germany

来源：

JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY | 2011年 / 127卷 / 02期

关键词：

Dendritic cells; Hymenoptera venom allergy; specific immunotherapy; flow cytometry; GRASS-POLLEN IMMUNOTHERAPY; IMMUNE-RESPONSES; ATOPIC-DERMATITIS; IN-VIVO; EFFICACY; DISEASE; INFLAMMATION; MECHANISMS; ACTIVATE; SUBSETS;

D O I：

10.1016/j.jaci.2010.12.003

中图分类号：

R392 [医学免疫学];

学科分类号：

100102 ;

摘要：

Background: Modulation of T-cell differentiation, which is controlled by dendritic cells (DCs), plays a crucial role in specific immunotherapy (SIT). However, the number and the characteristics of blood DCs before and during immunotherapy are unknown. Objective: To analyze the number and the characteristics of blood DC subsets in patients with Hymenoptera venom allergy before and after initiation of SIT. Methods: In this clinical trial (NCT00947908), blood myeloid and plasmacytoid DCs were analyzed in 20 patients with Hymenoptera venom allergy (bee or wasp venom) by using 4-color flow cytometry at 3 time points: directly before SIT, and 52 hours and 12 months after initiation of SIT. In addition, 20 age-matched and sex-matched controls were examined. Results: In patients with Hymenoptera venom allergy, the number of plasmacytoid DCs before SIT was comparable to that of controls. Plasmacytoid DCs decreased markedly 52 hours after initiation of SIT and returned to control levels after 12 months of treatment. Myeloid DCs were elevated in patients with Hymenoptera venom allergy before, during, and after the first 12 months of SIT. In addition, there were changes in the expression of function-associated surface molecules on myeloid DCs (such as Fc g receptor 2 and Toll-like receptor 2) during SIT. Conclusion: Numbers of blood myeloid DCs are elevated in patients with Hymenoptera venom allergy, and there are specific changes in the expression of function-associated surface molecules on these cells during SIT. Numbers of plasmacytoid DCs in blood are profoundly but are only transiently decreased after initiation of SIT. (J Allergy Clin Immunol 2011;127:487-94.)

引用

页码：487 / U1820

页数：11

共 37 条

[1] Role of interleukin 10 in specific immunotherapy [J].

Akdis, CA ;

Blesken, T ;

Akdis, M ;

Wüthrich, B ;

Blaser, K .

JOURNAL OF CLINICAL INVESTIGATION, 1998, 102 (01) :98-106

[2] Mechanisms and treatment of allergic disease in the big picture of regulatory T cells [J].

Akdis, Cezmi A. ;

Akdis, Muebeccel .

JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY, 2009, 123 (04) :735-746

[3] Immune responses in healthy and allergic individuals are characterized by a fine balance between allergen-specific T regulatory 1 and T helper 2 cells [J].

Akdis, M ;

Verhagen, J ;

Taylor, A ;

Karamloo, F ;

Karagiannidis, C ;

Crameri, R ;

Thunberg, S ;

Deniz, G ;

Valenta, R ;

Fiebig, H ;

Kegel, C ;

Disch, R ;

Schmidt-Weber, CB ;

Blaser, K ;

Akdis, CA .

JOURNAL OF EXPERIMENTAL MEDICINE, 2004, 199 (11) :1567-1575

[4] Functional expression of granzyme B in human plasmacytoid dendritic cells: a role in allergic inflammation [J].

Bratke, K. ;

Nielsen, J. ;

Manig, F. ;

Klein, C. ;

Kuepper, M. ;

Geyer, S. ;

Julius, P. ;

Lommatzsch, M. ;

Virchow, J. C. .

CLINICAL AND EXPERIMENTAL ALLERGY, 2010, 40 (07) :1015-1024

[5] Function-associated surface molecules on airway dendritic cells in cigarette smokers [J].

Bratke, Kai ;

Klug, Michael ;

Bier, Andrea ;

Julius, Peter ;

Kuepper, Michael ;

Virchow, J. Christian ;

Lommatzsch, Marek .

AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY, 2008, 38 (06) :655-660

[6] Dendritic cell subsets in human bronchoalveolar lavage fluid after segmental allergen challenge [J].

Bratke, Kai ;

Lommatzsch, Marek ;

Julius, Peter ;

Kuepper, Michael ;

Kleine, Hans-Dieter ;

Luttmann, Werner ;

Virchow, J. Christian .

THORAX, 2007, 62 (02) :168-175

[7] Efficacy of antihistamine pretreatment in the prevention of adverse reactions to Hymenoptera immunotherapy: A prospective, randomized, placebo-controlled trial [J].

Brockow, K ;

Kiehn, M ;

Riethmuller, C ;

Vieluf, D ;

Berger, J ;

Ring, J .

JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY, 1997, 100 (04) :458-463

[8] Early markers for protective mechanisms during rush venom immunotherapy [J].

Bussmann, C. ;

Xia, J. ;

Allam, J. -P. ;

Maintz, L. ;

Bieber, T. ;

Novak, N. .

ALLERGY, 2010, 65 (12) :1558-1565

[9] Ragweed immunotherapy in adult asthma [J].

Creticos, PS ;

Reed, CE ;

Norman, PS ;

Khoury, J ;

Adkinson, NF ;

Buncher, CR ;

Busse, WW ;

Bush, RK ;

Gadde, J ;

Li, JT ;

Richerson, HB ;

Rosenthal, RR ;

Solomon, WR ;

Steinberg, P ;

Yunginger, JW .

NEW ENGLAND JOURNAL OF MEDICINE, 1996, 334 (08) :501-506

[10] Essential role of lung plasmacytoid dendritic cells in preventing asthmatic reactions to harmless inhaled antigen [J].

de Heer, HJ ;

Hammad, H ;

Soullié, T ;

Hijdra, D ;

Vos, N ;

Willart, MAM ;

Hoogsteden, HC ;

Lambrecht, BN .

JOURNAL OF EXPERIMENTAL MEDICINE, 2004, 200 (01) :89-98

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