Dual-Signal Microbial Biosensor for the Detection of Dopamine without Inference from Other Catecholamine Neurotransmitters

被引：41

作者：

Lin, Yu-Kuan ^{[1
]}

Yeh, Yi-Chun ^{[1
]}

机构：

[1] Natl Taiwan Normal Univ, Dept Chem, 88,Sect 4,Tingzhou Rd, Taipei 11677, Taiwan

来源：

ANALYTICAL CHEMISTRY | 2017年 / 89卷 / 21期

关键词：

ESCHERICHIA-COLI; SENSITIVE DETECTION; NANOPARTICLES; ENZYME; 4,5-DIOXYGENASE; DISEASE;

D O I：

10.1021/acs.analchem.7b02498

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Dopamine, one of catecholamine neurotransmitters, plays an important role in many brain functions and behavioral responses. In this study, we developed a novel dual signal whole-cell biosensor for the detection of dopamine through the generation of red fluorescent proteins and 6-decarboxylated betaxanthin pigments. The proposed system responses specifically to dopamine with a detection limit of 1.43 mu M. Furthermore, a combination of dual output signals makes it possible to reduce the interference from other catecholamine neurotransmitters, including L-DOPA, epinephrine, and norepinephrine.

引用

页码：11178 / 11182

页数：5

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