Genome-wide analysis of 5-hydroxymethylcytosine distribution reveals its dual function in transcriptional regulation in mouse embryonic stem cells

被引：434

作者：

Wu, Hao ^{[2
,3
]}

D'Alessio, Ana C. ^{[1
,4
]}

Ito, Shinsuke ^{[1
,4
]}

Wang, Zhibin ^{[5
]}

Cui, Kairong ^{[6
]}

Zhao, Keji ^{[6
]}

Sun, Yi Eve ^{[2
,3
]}

Zhang, Yi ^{[1
,4
]}

机构：

[1] Univ N Carolina, Howard Hughes Med Inst, Chapel Hill, NC 27599 USA

[2] Univ Calif Los Angeles, David Geffen Sch Med, Dept Mol & Med Pharmacol, Los Angeles, CA 90095 USA

[3] Univ Calif Los Angeles, Semel Inst Neurosci & Human Behav, Intellectual Dev & Disabil Res Ctr, Dept Psychiat & Biobehav Sci, Los Angeles, CA 90095 USA

[4] Univ N Carolina, Lineberger Comprehens Canc Ctr, Dept Biochem & Biophys, Chapel Hill, NC 27599 USA

[5] Johns Hopkins Bloomberg Sch Publ Hlth, Dept Environm Hlth Sci, Lab Human Environm Epigenomes, Baltimore, MD 21025 USA

[6] NHLBI, Lab Mol Immunol, NIH, Bethesda, MD 20892 USA

来源：

GENES & DEVELOPMENT | 2011年 / 25卷 / 07期

关键词：

Tet1; 5-methylcytosine (5mC); 5-hydroxymethylcytosine (5hmC); mouse embryonic stem cells; genome-wide 5hmC distribution; Polycomb repression; DNA METHYLATION; PLURIPOTENT; CONVERSION; MAPS;

D O I：

10.1101/gad.2036011

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Recent studies have demonstrated that the Ten-eleven translocation (Tet) family proteins can enzymatically convert 5-methylcytosine (5mC) to 5-hydroxymethylcytosine (5hmC). While 5mC has been studied extensively, little is known about the distribution and function of 5hmC. Here we present a genome-wide profile of 5hmC in mouse embryonic stem (ES) cells. A combined analysis of global 5hmC distribution and gene expression profile in wild-type and Tet1-depleted ES cells suggests that 5hmC is enriched at both gene bodies of actively transcribed genes and extended promoter regions of Polycomb-repressed developmental regulators. Thus, our study reveals the first genome-wide 5hmC distribution in pluripotent stem cells, and supports its dual function in regulating gene expression.

引用

页码：679 / 684

页数：6

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