Bioactive endophytic fungi isolated from Caesalpinia echinata Lam. (Brazilwood) and identification of beauvericin as a trypanocidal metabolite from Fusarium sp.

被引:33
|
作者
Campos, Fernanda Fraga [1 ,2 ]
Sales Junior, Policarpo A. [2 ]
Romanha, Alvaro Jose [2 ,3 ]
Araujo, Marcio S. S. [2 ]
Siqueira, Ezequias P. [2 ]
Resende, Jarbas M. [4 ]
Alves, Tania M. A. [2 ]
Martins-Filho, Olindo A. [2 ]
dos Santos, Vera Lucia [5 ]
Rosa, Carlos A. [5 ]
Zani, Carlos L. [2 ]
Cota, Betania Barros [2 ]
机构
[1] Univ Fed Vales Jequitinhonha & Mucuri, Dept Ciencias Biol & Saude, Diamantina, MG, Brazil
[2] Ctr Pesquisas Rene Rachou Fiocruz, Belo Horizonte, MG, Brazil
[3] Univ Fed Santa Catarina, Dept Microbiol Imunol & Parasitol, Florianopolis, SC, Brazil
[4] Univ Fed Minas Gerais, Inst Ciencias Exatas, Dept Quim, Belo Horizonte, MG, Brazil
[5] Univ Fed Minas Gerais, Inst Ciencias Biol, Dept Microbiol, Belo Horizonte, MG, Brazil
来源
MEMORIAS DO INSTITUTO OSWALDO CRUZ | 2015年 / 110卷 / 01期
关键词
endophytic fungi; bioactive; Caesalpinia echinata Lam; Fabaceae; Trypanosoma cruzi; beauvericin; ANTIMICROBIAL ACTIVITY; IN-VITRO; DIVERSITY; ENNIATINS; FUSAPROLIFERIN; MYCOTOXINS; INHIBITORS; CELLS;
D O I
10.1590/0074-02760140243
中图分类号
R38 [医学寄生虫学]; Q [生物科学];
学科分类号
07 ; 0710 ; 09 ; 100103 ;
摘要
Aiming to identify new sources of bioactive secondary metabolites, we isolated 82 endophytic fungi from stems and barks of the native Brazilian tree Caesalpinia echinata Lam. (Fabaceae). We tested their ethyl acetate extracts in several in vitro assays. The organic extracts from three isolates showed antibacterial activity against Staphylococcus aureus and Escherichia coli [minimal inhibitory concentration (MIC) 32-64 mu g/mL]. One isolate inhibited the growth of Salmonella typhimurium (MIC 64 mu g/mL) and two isolates inhibited the growth of Klebsiella oxytoca (MIC 64 mu g/mL), Candida albicans and Candida tropicalis (MIC 64-128 mu g/mL). Fourteen extracts at a concentration of 20 mu g/mL showed antitumour activities against human breast cancer and human renal cancer cells, while two isolates showed anti-tumour activities against human melanoma cancer cells. Six extracts were able to reduce the proliferation of human peripheral blood mononuclear cells, indicating some degree of selective toxicity. Four isolates were able to inhibit Leishmania (Leishmania) amazonensis and one isolate inhibited Trypanosoma cruzi by at least 40% at 20 mu g/mL. The trypanocidal extract obtained from Fusarium sp. [KF611679] culture was subjected to bioguided fractionation, which revealed beauvericin as the compound responsible for the observed toxicity of Fusarium sp. to T. cruzi. This depsipeptide showed a half maximal inhibitory concentration of 1.9 mu g/mL (2.43 mu M) in a T. cruzi cellular culture assay.
引用
收藏
页码:65 / 74
页数:10
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