A linear pentapeptide is a quorum-sensing factor required for mazEF-mediated cell death in Escherichia coli

被引：200
作者：
Kolodkin-Gal, Ilana
Hazan, Ronen
Gaathon, Ariel
Carmeli, Shmuel
Engelberg-Kulka, Hanna [1 ]
机构：
[1] Hebrew Univ Jerusalem, Hadassah Med Sch, Dept Mol Biol, IL-91120 Jerusalem, Israel
[2] Hebrew Univ Jerusalem, Hadassah Med Sch, Core Res Facil, IL-91120 Jerusalem, Israel
[3] Tel Aviv Univ, Sch Chem, IL-69978 Tel Aviv, Israel
来源：
SCIENCE | 2007年 / 318卷 / 5850期
关键词：
D O I：
10.1126/science.1147248
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
mazEF is a toxin-antitoxin module located on many bacterial chromosomes, including those of pathogens. Here, we report that Escherichia coli mazEF-mediated cell death is a population phenomenon requiring a quorum-sensing molecule that we call the extracellular death factor (EDF). Structural analysis revealed that EDF is a linear pentapeptide, Asn-Asn-Trp-Asn-Asn. Each of the five amino acids of EDF is important for its activity.
引用
收藏
页码：652 / 655
页数：4
相关论文
共 27 条
[1]   An Escherichia coli chromosomal ''addiction module'' regulated by 3',5'-bispyrophosphate: A model for programmed bacterial cell death [J].
Aizenman, E ;
EngelbergKulka, H ;
Glaser, G .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1996, 93 (12) :6059-6063
[2]   Small talk: Cell-to-cell communication in bacteria [J].
Bassler, BL .
CELL, 2002, 109 (04) :421-424
[3]   Bacterially speaking [J].
Bassler, BL ;
Losick, R .
CELL, 2006, 125 (02) :237-246
[4]   Cell death: Critical control points [J].
Danial, NN ;
Korsmeyer, SJ .
CELL, 2004, 116 (02) :205-219
[5]   A three-protein signaling pathway governing immunity to a bacterial cannibalism toxin [J].
Ellermeier, CD ;
Hobbs, EC ;
Gonzalez-Pastor, JE ;
Losick, R .
CELL, 2006, 124 (03) :549-559
[6]   mazEF:: a chromosomal toxin-antitoxin module that triggers programmed cell death in bacteria [J].
Engelberg-Kulka, H ;
Hazan, R ;
Amitai, S .
JOURNAL OF CELL SCIENCE, 2005, 118 (19) :4327-4332
[7]   Bacterial programmed cell death and multicellular behavior in bacteria [J].
Engelberg-Kulka, Hanna ;
Amitai, Shahar ;
Kolodkin-Gal, Ilana ;
Hazan, Ronen .
PLOS GENETICS, 2006, 2 (10) :1518-1526
[8]   Census and consensus in bacterial ecosystems: The LuxR-LuxI family of quorum-sensing transcriptional regulators [J].
Fuqua, C ;
Winans, SC ;
Greenberg, EP .
ANNUAL REVIEW OF MICROBIOLOGY, 1996, 50 :727-751
[9]   Cannibalism by sporulating bacteria [J].
Gonzalez-Pastor, JE ;
Hobbs, EC ;
Losick, R .
SCIENCE, 2003, 301 (5632) :510-513
[10]   Toxins-antitoxins: Plasmid maintenance, programmed cell death, and cell cycle arrest [J].
Hayes, F .
SCIENCE, 2003, 301 (5639) :1496-1499
123