Structural elucidation, chain conformation and immuno-modulatory activity of glucogalactomannan from cultured Cordyceps sinensis fungus UM01

被引：45
作者：
Cheong, Kit-Leong [1 ]
Meng, Lan-Zhen [1 ]
Chen, Xian-Qiang [1 ]
Wang, Lan-Ying [1 ]
Wu, Ding-Tao [1 ]
Zhao, Jing [1 ]
Li, Shao-Ping [1 ]
机构：
[1] Univ Macau, Inst Chinese Med Sci, State Key Lab Qual Res Chinese Med, Macau, Peoples R China
来源：
JOURNAL OF FUNCTIONAL FOODS | 2016年 / 25卷
基金：
中国国家自然科学基金;
关键词：
Cordyceps sinensis; Polysaccharide; Chain conformation; Chemical structural; Immuno-modulatory activity; TRADITIONAL CHINESE MEDICINE; HYPERBRANCHED POLYSACCHARIDE; ACIDIC POLYSACCHARIDE; MACROPHAGES; CHROMATOGRAPHY; CELLS; FRUCTOOLIGOSACCHARIDES; OLIGOSACCHARIDES; ANTIOXIDATION; EXPRESSION;
D O I：
10.1016/j.jff.2016.06.002
中图分类号：
TS2 [食品工业];
学科分类号：
0832 ;
摘要：
The chemical structure, chain conformation, and immuno-modulatory activity of polysaccharide from mycelium Cordyceps sinensis fungus UM01 were investigated. The molecular weights and the intrinsic viscosity of purified polysaccharide (UM01-S4) were determined as 22,559 Da and 5.09 mL/g, respectively. Using the polymer solution theory, UM01-S4 has a sphere-like conformation in aqueous NaCl at 35 degrees C. The chemical structure of UM01-S4 contains an alpha-(1 -> 2)-Manp core. The side chains were composed of beta-(1 -> 2)-Galf, beta-(1 -> 4)-Glcp, alpha-GalpA, and alpha-Manp units, which attached to the mannan core at the O-6 position. The immuno-modulatory assays showed that UM01-S4 exhibited good activities, including macrophages proliferation, phagocytosis, and release of NO and cytokines. The mechanism underlying the macrophage regulation of UM01-S4 might be related to the activation of the MAPK and NF-kappa B signalling pathways. Thus, mycelium of C. sinensis UM01 could be used as a natural immune-stimulating agent in functional food areas and pharmaceutical industries. (C) 2016 Elsevier Ltd. All rights reserved.
引用
收藏
页码：174 / 185
页数：12
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