Downy mildew (Pl 8 and Pl 14 ) and rust (R Adv ) resistance genes reside in close proximity to tandemly duplicated clusters of non-TIR-like NBS-LRR-encoding genes on sunflower chromosomes 1 and 13

被引:44
作者
Bachlava, Eleni [1 ,2 ]
Radwan, Osman E. [1 ,3 ]
Abratti, Gustavo [4 ,5 ]
Tang, Shunxue [1 ,6 ]
Gao, Wenxiang [1 ,6 ]
Heesacker, Adam F. [1 ]
Bazzalo, Maria E. [4 ]
Zambelli, Andres [4 ]
Leon, Alberto J. [4 ]
Knapp, Steven J. [1 ,2 ]
机构
[1] Univ Georgia, Inst Plant Breeding Genet & Genom, Athens, GA 30602 USA
[2] Monsanto Co, Woodland, CA 95695 USA
[3] Univ Illinois, Urbana, IL 61801 USA
[4] Advanta Seeds, Balcarce Res Stn Ruta 226, Balcarce PCIA Buenos Aires, Buenos Aires, DF, Argentina
[5] Pioneer Hibred, Buenos Aires, DF, Argentina
[6] Dow AgroSci LLC, Indianapolis, IN 46268 USA
基金
美国国家科学基金会; 美国农业部;
关键词
MARKER-ASSISTED SELECTION; HELIANTHUS-ANNUUS L; DISEASE-RESISTANCE; PLASMOPARA-HALSTEDII; PL5/PL8; LOCUS; IDENTIFICATION; EVOLUTION; LINKAGE; REGISTRATION; INHERITANCE;
D O I
10.1007/s00122-010-1525-0
中图分类号
S3 [农学(农艺学)];
学科分类号
0901 ;
摘要
Nucleotide binding site-leucine rich repeat (NBS-LRR) proteins are encoded by a ubiquitous gene family in sunflower and frequently harbor disease resistance genes. We investigated NBS-LRR-encoding resistance gene candidates (RGCs) flanking the downy mildew resistance genes Pl (8) and Pl (14) and the rust resistance gene R (Adv) , which map on the NBS-LRR clusters of linkage groups 1 and 13 in sunflower genome. We shotgun sequenced bacterial artificial chromosome (BAC) clones proximal to Pl (8) , Pl (14) , and R (Adv) and identified seven novel non-Toll/interleukin-1 receptor (TIR)-like NBS-LRR RGCs, which clustered with previously identified RGCs of linkage group 13 but were phylogenetically distant from the TIR- and non-TIR-NBS-LRR-encoding superfamilies of sunflower. Six of the seven predicted RGCs have intact open reading frames and reside in genomic segments with abundant transposable elements. The genomic localization and sequence similarity of the novel non-TIR-like predicted RGCs suggests that they originated from tandem duplications. RGCs in the proximity of Pl (8) and R (Adv) were likely introgressed from silverleaf sunflower genome, where the RGC cluster of linkage group 13 is duplicated in two independent chromosomes that have different architecture and level of recombination from the respective common sunflower chromosomes.
引用
收藏
页码:1211 / 1221
页数:11
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