3-Dimensional reconstruction of fluorescent structures in tardigrades

被引：7

作者：

Pfannkuchen, Martin ^{[1
]}

Bruemmer, Franz ^{[1
]}

Schill, Ralph O. ^{[1
]}

机构：

[1] Univ Stuttgart, Inst Biol, Dept Zool, D-70569 Stuttgart, Germany

来源：

JOURNAL OF LIMNOLOGY | 2007年 / 66卷

关键词：

structured illumination; optical sectioning; fluorescence microscopy; ApoTome; 3-dimensional rendering; Tardigrada; measuring 3D structures;

D O I：

10.4081/jlimnol.2007.s1.158

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Tardigrades are microscopic animals, thus bri&field microscopy is a well established method for tardigrade observation. Modern techniques in functional genetics like fluorescence in situ hybridisation or fluorescently labelled expression markers demand high resolution fluorescence microscopy. Nevertheless fardigrades are still considered to be difficult objects for fluorescence techniques as they are covered by an opaque and diffracting cuticle. We show a modern technique of structured light illumination that enables us to acquire thin optical sections and consequently to reconstruct 3-dimensional structures in tardigrades with a high spatial resolution in all 3 dimensions. This technique is evaluated on taxonomically valuable internal as well as external structures of eutardigrades: the bucco-pharyngeal apparatus and the claws. The 3-dimensional reconstructions allow the measurement of distances in all 3 dimensions.

引用

页码：158 / 163

页数：6

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