Antibacterial Defense of Human Airway Epithelial Cells from Chronic Obstructive Pulmonary Disease Patients Induced by Acute Exposure to Nontypeable Haemophilus influenzae: Modulation by Cigarette Smoke

被引:48
|
作者
Amatngalim, Gimano D. [1 ]
Schrumpf, Jasmijn A. [1 ]
Henic, Almira [1 ]
Dronkers, Esther [1 ]
Verhoosel, Renate M. [1 ]
Ordonez, Soledad R. [3 ]
Haagsman, Henk P. [3 ]
Fuentes, Maria E. [4 ]
Sridhar, Sriram [4 ]
Aarbiou, Jamil [2 ]
Janssen, Richard A. J. [2 ]
Lekkerkerker, Annemarie N. [2 ]
Hiemstra, Pieter S. [1 ]
机构
[1] Leiden Univ, Med Ctr, Dept Pulmonol, Albinusdreef 2, NL-2333 ZA Leiden, Netherlands
[2] Galapagos BV, Leiden, Netherlands
[3] Univ Utrecht, Fac Vet Med, Dept Infect Dis & Immunol, Div Mol Host Def, Utrecht, Netherlands
[4] Hoffmann La Roche, Nutley, NJ USA
关键词
Antimicrobial peptides; Epithelium; Host defense; Chronic obstructive pulmonary disease; Cigarette smoke; FACTOR-KAPPA-B; CYSTIC-FIBROSIS; HOST-DEFENSE; ANTIMICROBIAL PEPTIDES; HUMAN BETA-DEFENSIN-2; BACTERIAL-INFECTION; SIGNALING PATHWAY; INNATE IMMUNITY; P38; MAPK; IN-VITRO;
D O I
10.1159/000455193
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Antimicrobial proteins and peptides (AMPs) are a central component of the antibacterial activity of airway epithelial cells. It has been proposed that a decrease in antibacterial lung defense contributes to an increased susceptibility to microbial infection in smokers and patients with chronic obstructive pulmonary disease (COPD). However, whether reduced AMP expression in the epithelium contributes to this lower defense is largely unknown. We investigated the bacterial killing activity and expression of AMPs by air-liquid interface-cultured primary bronchial epithelial cells from patients and non-COPD (ex-)smokers that were stimulated with nontypeable Haemophilus influenzae (NTHi). In addition, the effect of cigarette smoke on AMP expression and the activation of signaling pathways was determined. COPD cell cultures displayed reduced antibacterial activity, whereas smoke exposure suppressed the NTHi-induced expression of AMPs and further increased IL-8 expression in COPD and nonCOPD cultures. Moreover, smoke exposure impaired NTHi-induced activation of NF-KB, but not MAP-kinase signaling. Our findings demonstrate that the antibacterial activity of cultured airway epithelial cells induced by acute bacterial exposure was reduced in COPD and suppressed by cigarette smoke, whereas inflammatory responses persisted. These findings help to explain the imbalance between protective antibacterial and destructive inflammatory innate immune responses in COPD. (C) 2017 The Author(s) Published by S. Karger AG, Basel.
引用
收藏
页码:359 / 374
页数:16
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