Size fractionation of bacterial capsular polysaccharides for their use in conjugate vaccines

被引：52
|
作者：
Costantino, P
Norelli, F
Giannozzi, A
D'Ascenzi, S
Bartoloni, A
Kaur, S
Tang, DZ
Seid, R
Viti, S
Paffetti, R
Bigio, M
Pennatini, C
Averani, G
Guarnieri, V
Gallo, E
Ravenscroft, N
Lazzeroni, C
Rappuoli, R
Ceccarini, C
机构：
[1] Chiron Vaccines SpA, I-53100 Siena, Italy
[2] Chiron Corp, Emeryville, CA 94608 USA
来源：
VACCINE | 1999年 / 17卷 / 9-10期
关键词：
conjugate vaccines; capsular polysaccharide; oligosaccharide; intermediate chain length oligomers; hydrolysis; size fractionation;
D O I：
10.1016/S0264-410X(98)00348-X
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
We have developed a chromatographic method suitable for the fractionation of polysaccharides having a negatively charged group. The method permits the removal of all those polysaccharide fragments having a short sequence and which are likely unsuitable for conjugate Vaccine construction. The selected polysaccharide fragments can be used to produce glycoconjugate vaccines containing a restricted saccharide polydispersion. We have applied this chromatographic method to three different antigens, Haemophilus influenzae type b and Neisseria meningitidis group A and group C polysaccharides. The method is easily adapted for manufacturing purposes. (C) 1999 Elsevier Science Ltd. All rights reserved.
引用
收藏
页码：1251 / 1263
页数：13
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