Pavlova sp. was employed to evaluate the efficiency of different lipid extraction methods. The microalgal crude lipids content determined using the mixed solvent with ultrasonic method was 44.7 wt.%. The triglyceride content obtained by the mixed solvent method was 15.6 wt.%. The extraction yield was the FAME yield divided by the maximum FAME (15.9 wt.%). The extraction yield was improved by cell disruption prior to extraction, and the highest triglyceride extraction yield of 98.7% was observed using the supercritical fluid extraction (SFE) method with bead-beating. The results indicate that the SFE method is effective and provides higher selectivity for triglyceride extraction though the total lipid extracted was less than that using solvent extraction. (C) 2011 Elsevier Ltd. All rights reserved.
机构:
Chinese Univ Hong Kong, Dept Biol, Shatin, New Territories, Peoples R ChinaChinese Univ Hong Kong, Dept Biol, Shatin, New Territories, Peoples R China
机构:
Virginia Polytech Inst & State Univ, Dept Biol Syst Engn, Blacksburg, VA 24061 USAVirginia Polytech Inst & State Univ, Dept Biol Syst Engn, Blacksburg, VA 24061 USA
Johnson, Michael B.
;
Wen, Zhiyou
论文数: 0引用数: 0
h-index: 0
机构:
Virginia Polytech Inst & State Univ, Dept Biol Syst Engn, Blacksburg, VA 24061 USAVirginia Polytech Inst & State Univ, Dept Biol Syst Engn, Blacksburg, VA 24061 USA
机构:
Chinese Univ Hong Kong, Dept Biol, Shatin, New Territories, Peoples R ChinaChinese Univ Hong Kong, Dept Biol, Shatin, New Territories, Peoples R China
机构:
Virginia Polytech Inst & State Univ, Dept Biol Syst Engn, Blacksburg, VA 24061 USAVirginia Polytech Inst & State Univ, Dept Biol Syst Engn, Blacksburg, VA 24061 USA
Johnson, Michael B.
;
Wen, Zhiyou
论文数: 0引用数: 0
h-index: 0
机构:
Virginia Polytech Inst & State Univ, Dept Biol Syst Engn, Blacksburg, VA 24061 USAVirginia Polytech Inst & State Univ, Dept Biol Syst Engn, Blacksburg, VA 24061 USA