Isolation and Characterization of Sixteen Polymorphic Microsatellite Loci in the Golden Apple Snail Pomacea canaliculata

被引：12

作者：

Chen, Lian ^{[1
]}

Xu, Haigen ^{[1
]}

Li, Hong ^{[2
]}

Wu, Jun ^{[1
]}

Ding, Hui ^{[1
]}

Liu, Yan ^{[1
]}

机构：

[1] Minist Environm Protect, Nanjing Inst Environm Sci, Nanjing 210042, Jiangsu, Peoples R China

[2] Nanjing Normal Univ, Jiangsu Key Lab Biodivers & Biotechnol, Coll Life Sci, Nanjing 210046, Jiangsu, Peoples R China

来源：

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | 2011年 / 12卷 / 09期

基金：

中国国家自然科学基金;

关键词：

golden apple snail; invasive species; microsatellite loci; Pomacea canaliculata; NULL ALLELES; SOFTWARE;

D O I：

10.3390/ijms12095993

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We report the characterization of 16 polymorphic microsatellite markers in the golden apple snail, Pomacea canaliculata, a pest registered in the list of "100 of the world's worst invasive alien species". The fast isolation by AFLP (Amplified Fragment Length Polymorphism) of sequences containing repeats (FIASCO) method was used to isolate microsatellite loci, and polymorphism was explored with 29 individuals collected in an invasive region from China. These primers showed a number of alleles per locus ranging from three to 13. The ranges of observed and expected heterozygosity were 0.310-0.966 and 0.523-0.898, respectively. These microsatellite markers described here will be useful for population genetic studies of P. canaliculata.

引用

页码：5993 / 5998

页数：6

共 23 条

[1] A method for genotype validation and primer assessment in heterozygote-deficient species, as demonstrated in the prosobranch mollusc Hydrobia ulvae [J].

Brownlow, Robert J. ;

Dawson, Deborah A. ;

Horsburgh, Gavin J. ;

Bell, James J. ;

Fish, John D. .

BMC GENETICS, 2008, 9 (1)

[2] Effects of Microsatellite Null Alleles on Assignment Testing [J].

Carlsson, Jens .

JOURNAL OF HEREDITY, 2008, 99 (06) :616-623

[3] Wild and aquaculture populations of the eastern oyster compared using microsatellites [J].

Carlsson, Jens ;

Morrison, Cheryl L. ;

Reece, Kimberly S. .

JOURNAL OF HEREDITY, 2006, 97 (06) :595-598

[4] Microsatellite null alleles and estimation of population differentiation [J].

Chapuis, Marie-Pierre ;

Estoup, Arnaud .

MOLECULAR BIOLOGY AND EVOLUTION, 2007, 24 (03) :621-631

[5] Characterization and PCR multiplexing of polymorphic microsatellite loci for the locust Locusta migratoria [J].

Chapuis, MP ;

Loiseau, A ;

Michalakis, Y ;

Lecoq, M ;

Estoup, A .

MOLECULAR ECOLOGY NOTES, 2005, 5 (03) :554-557

[6]

Cowie R. H., 2002, Molluscs as crop pests, P145, DOI 10.1079/9780851993201.0145

[7] Microsatellite null alleles in parentage analysis [J].

Dakin, EE ;

Avise, JC .

HEREDITY, 2004, 93 (05) :504-509

[8] Commentary : Experimental verification of microsatellite null alleles in norway spruce (Picea abies [L.] Karst.): Implications for population genetic studies [J].

De Sousa S.N. ;

Finkeldey R. ;

Gailing O. .

Plant Molecular Biology Reporter, 2005, 23 (2) :113-119

[9] Out of South America: multiple origins of non-native apple snails in Asia [J].

Hayes, K. A. ;

Joshi, R. C. ;

Thiengo, S. C. ;

Cowie, R. H. .

DIVERSITY AND DISTRIBUTIONS, 2008, 14 (04) :701-712

[10]

Hu Y.C., 2010, CHIN AGR SCI B, V26, P245

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