Requirement of mammalian Timeless for circadian rhythmicity

被引：161
作者：
Barnes, JW
Tischkau, SA
Barnes, JA
Mitchell, JW
Burgoon, PW
Hickok, JR
Gillette, MU [1 ]
机构：
[1] Univ Illinois, Dept Cell & Struct Biol, Urbana, IL 61801 USA
[2] Univ Illinois, Dept Vet Biosci, Urbana, IL 61802 USA
来源：
SCIENCE | 2003年 / 302卷 / 5644期
关键词：
D O I：
10.1126/science.1086593
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Despite a central circadian role in Drosophila for the transcriptional regulator Timeless (dTim), the relevance of mammalian Timeless (mTim) remains equivocal. Conditional knockdown of mTim protein expression in the rat suprachiasmatic nucleus (SCN) disrupted SCN neuronal activity rhythms, and altered levels of known core clock elements. Full-length mTim protein (mTIM-fl) exhibited a 24-hour oscillation, whereas a truncated isoform (mTIM-s) was constitutively expressed. mTIM-fl associated with the mammalian clock Period proteins (mPERs) in oscillating SCN cells. These data suggest that mTim is required for rhythmicity and is a functional homolog of dTim on the negative-feedback arm of the mammalian molecular clockwork.
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页码：439 / 442
页数：4
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