Biological wastewater treatment is a promising and environmentally friendly method that utilises living microorganisms to remediate water and enable recovery or conversion of contaminants into valuable products. For many decades, microalgae and cyanobacteria, photosynthetic living microorganisms, have been explored extensively for wastewater bioremediation. They can be used for recovering valuable nutrients such as nitrogen and phosphorous from secondary effluents and capable of transforming those nutrients into marketable products such as biofuels, biofertilisers, nutraceutical, and pigments for promoting a Bio-Circular Green economy. In recent years, there has been a shift towards mixing compatible microalgae with bacteria, which is inspired by their natural symbiotic relationships to increase nitrogen and phosphorus recoveries. With this enhanced bioremediation, recovery of polluted wastes can be intensified and higher biomass quality (with high nutrient density) can be achieved. This review focuses on the state-of-the-art of mixed microalgal-bacterial cultivating systems. A comprehensive comparison of existing studies that used Chlorella species as microalgae in various mixed microalgal-bacterial cultivating systems (suspension, biofilm, and immobilisation) for nitrogen and phosphorus recoveries from wastewater is conducted. Key technical challenges such as balancing microalgae and bacteria species, pH regulation, light distribution, biomass harvesting, and biomass conversion are also discussed. From the data comparisons among different cultivation systems, it has been suggested that immobilisation appears to require less amount of operational light compared to the suspended and biofilm-based systems for similar nitrogen and phosphorus removal efficiencies.
