Genotypic analysis of Escherichia coli strains from poultry carcasses and their susceptibilities to antimicrobial agents

被引:25
作者
Geornaras, I
Hastings, JW
von Holy, A
机构
[1] Univ Witwatersrand, Dept Mol & Cell Biol, ZA-2050 Johannesburg, South Africa
[2] Univ KwaZulu Natal, Sch Mol & Cellular Biosci, ZA-3209 Scottsville, South Africa
关键词
D O I
10.1128/AEM.67.4.1940-1944.2001
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Plasmid profiling and amplified fragment length polymorphism (AFLP) analysis were used to genotype 50 Escherichia coli strains from poultry carcasses. Thirty different plasmid profiles were evident, and clustering of the AFLP data showed that they were a distinctly heterogeneous group of strains. Susceptibility testing against five antimicrobial agents used in the South African poultry industry showed all strains to be susceptible to danofloxacin and colistin, while the majority (96%) were resistant to two tetracyclines.
引用
收藏
页码:1940 / 1944
页数:5
相关论文
共 38 条
[1]   Molecular characterization of Escherichia coli strains isolated from pigs with edema disease [J].
Aarestrup, FM ;
Jorsal, SE ;
Ahrens, P ;
Jensen, NE ;
Meyling, A .
JOURNAL OF CLINICAL MICROBIOLOGY, 1997, 35 (01) :20-24
[2]  
Aarts HJM, 1998, LETT APPL MICROBIOL, V26, P131, DOI 10.1046/j.1472-765X.1998.00302.x
[3]   SIMPLE AND RAPID METHOD FOR ISOLATING LARGE PLASMID DNA FROM LACTIC STREPTOCOCCI [J].
ANDERSON, DG ;
MCKAY, LL .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1983, 46 (03) :549-552
[4]   Avoparcin used as a growth promoter is associated with the occurrence of vancomycin-resistant Enterococcus faecium on Danish poultry and pig farms [J].
Bager, F ;
Madsen, M ;
Christensen, J ;
Aarestrup, FM .
PREVENTIVE VETERINARY MEDICINE, 1997, 31 (1-2) :95-112
[5]   FARM-ANIMALS AS A PUTATIVE RESERVOIR FOR VANCOMYCIN-RESISTANT ENTEROCOCCAL INFECTION IN MAN [J].
BATES, J ;
JORDENS, JZ ;
GRIFFITHS, DT .
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 1994, 34 (04) :507-514
[6]   Prevalence of bacterial resistance to quinolones and other antimicrobials among avian Escherichia coli strains isolated from septicemic and healthy chickens in Spain [J].
Blanco, JE ;
Blanco, M ;
Mora, A ;
Blanco, J .
JOURNAL OF CLINICAL MICROBIOLOGY, 1997, 35 (08) :2184-2185
[7]   Resistance responses of microorganisms in food environments [J].
Bower, CK ;
Daeschel, MA .
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, 1999, 50 (1-2) :33-44
[8]   High-resolution genotyping of Streptococcus pyogenes serotype M1 isolates by fluorescent amplified-fragment length polymorphism analysis [J].
Desai, M ;
Efstratiou, A ;
George, R ;
Stanley, J .
JOURNAL OF CLINICAL MICROBIOLOGY, 1999, 37 (06) :1948-1952
[9]  
Duim B, 1999, APPL ENVIRON MICROB, V65, P2369
[10]   QUINOLONE RESISTANCE IN CAMPYLOBACTER ISOLATED FROM MAN AND POULTRY FOLLOWING THE INTRODUCTION OF FLUOROQUINOLONES IN VETERINARY-MEDICINE [J].
ENDTZ, HP ;
RUIJS, GJ ;
VANKLINGEREN, B ;
JANSEN, WH ;
VANDERREYDEN, T ;
MOUTON, RP .
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 1991, 27 (02) :199-208