Dopamine sensing upon amphetamine administration

Anti-psychotic drugs and its effects on various neurotransmitters have been studied tremendously in the past few years. Here, we study the effect of Amphetamine (AMPH), both an addictive and therapeutic drug on Dopamine (DA) release. We administer AMPH drug of high dosage 10mg/kg to an adult male Sprague-Dawley rat (similar to 350-400g) followed by electrical stimulation of DA neuron. The current corresponding to the DA concentration was sensed by a carbon fiber electrode encased in a glass. The current resulted from oxidation of DA was observed for varying stimulation lengths. Principal component regression (PCR), a multivariate two-step multivariate calibration method that involves principal component analysis (PCA) followed by regression, which was used to analyze the data. The presence of the outliers introduced 20x error in the experimentally data and PCR predicted DA concentration. Outliers were thus detected using Cook's distance algorithm and eliminated from the training set. The improved PCR prediction shows almost accurate DA concentration prediction with linearity of 0.999.