Live-attenuated RNA hybrid vaccine technology provides single-dose protection against Chikungunya virus

被引：13
作者：
Voigt, Emily A. [1 ]
Fuerte-Stone, Jasmine [1 ]
Granger, Brian [1 ]
Archer, Jacob [1 ]
Van Hoeven, Neal [1 ,2 ]
机构：
[1] Infect Dis Res Inst, 1616 Eastlake Ave East,Suite 400, Seattle, WA 98102 USA
[2] PAI Life Sci, 1616 Eastlake Ave East, Seattle, WA 98102 USA
来源：
MOLECULAR THERAPY | 2021年 / 29卷 / 09期
基金：
美国国家卫生研究院;
关键词：
YELLOW-FEVER; IMMUNOGENICITY; CHALLENGES; DELIVERY; IMMUNITY; STRAIN; SAFETY;
D O I：
10.1016/j.ymthe.2021.05.018
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
We present a live-attenuated RNA hybrid vaccine technology that uses an RNA vaccine delivery vehicle to deliver in vitro -transcribed, full-length, live-attenuated viral genomes to the site of vaccination. This technology allows ready manufacturing in a cell-free environment, regardless of viral attenuation level, and it promises to avoid many safety and manufacturing challenges of traditional live-attenuated vaccines. We demonstrate this technology through development and testing of a live -attenuated RNA hybrid vaccine against Chikungunya virus (CHIKV), comprised of an in vitro-transcribed, highly attenuated CHIKV genome delivered by a highly stable nanostructured lipid carrier (NLC) formulation as an intramuscular injection. We demonstrate that single-dose immunization of immunocompetent C57BL/6 mice results in induction of high CHIKV-neutralizing antibody titers and protection against mortality and footpad swelling after lethal CHIKV challenge.
引用
收藏
页码：2782 / 2793
页数：12
相关论文
共 50 条
  • [21] Immunogenicity & safety of a single dose of live-attenuated Japanese encephalitis vaccine SA 14-14-2 in adults
    Khan, Siraj Ahmed
    Kakati, Sanjeeb
    Dutta, Prafulla
    Chowdhury, Purvita
    Borah, Jani
    Topno, Rashmee
    Jadhav, Santoshkumar M.
    Mohapatra, Pradyumna K.
    Mahanta, Jagadish
    Gupte, Mohan D.
    INDIAN JOURNAL OF MEDICAL RESEARCH, 2016, 144 : 886 - 892
  • [22] A single-dose ChAdOx1-vectored vaccine provides complete protection against Nipah Bangladesh and Malaysia in Syrian golden hamsters
    van Doremalen, Neeltje
    Lambe, Teresa
    Sebastian, Sarah
    Bushmaker, Trenton
    Fischer, Robert
    Feldmann, Friederike
    Haddock, Elaine
    Letko, Michael
    Avanzato, Victoria A.
    Rissanen, Ilona
    LaCasse, Rachel
    Scott, Dana
    Bowden, Thomas A.
    Gilbert, Sarah
    Munster, Vincent
    PLOS NEGLECTED TROPICAL DISEASES, 2019, 13 (06):
  • [23] A VLP-based vaccine provides complete protection against Nipah virus challenge following multiple-dose or single-dose vaccination schedules in a hamster model
    Walpita, Pramila
    Cong, Yu
    Jahrling, Peter B.
    Rojas, Oscar
    Postnikova, Elena
    Yu, Shuiqing
    Johns, Lisa
    Holbrook, Michael. R.
    NPJ VACCINES, 2017, 2
  • [24] The disruption of LPS in Salmonella Typhimurium provides partial protection against Salmonella Choleraesuis as a live attenuated vaccine
    Zhou, Guodong
    Ma, Qifeng
    Li, Quan
    Wang, Shifeng
    Shi, Huoying
    VACCINE, 2023, 41 (41) : 5951 - 5956
  • [25] Improved mucosal protection against Venezuelan equine encephalitis virus is induced by the molecularly defined, live-attenuated V3526 vaccine candidate
    Hart, MK
    Caswell-Stephan, K
    Bakken, R
    Tammariello, R
    Pratt, W
    Davis, N
    Johnston, RE
    Smith, J
    Steele, K
    VACCINE, 2000, 18 (26) : 3067 - 3075
  • [26] Protection induced by commercially available live-attenuated and recombinant viral vector vaccines against infectious laryngotracheitis virus in broiler chickens
    Vagnozzi, Ariel
    Zavala, Guillermo
    Riblet, Sylva M.
    Mundt, Alice
    Garcia, Maricarmen
    AVIAN PATHOLOGY, 2012, 41 (01) : 21 - 31
  • [27] Single-dose attenuated Vesiculovax vaccines protect primates against Ebola Makona virus
    Mire, Chad E.
    Matassov, Demetrius
    Geisbert, Joan B.
    Latham, Theresa E.
    Agans, Krystle N.
    Xu, Rong
    Ota-Setlik, Ayuko
    Egan, Michael A.
    Fenton, Karla A.
    Clarke, David K.
    Eldridge, John H.
    Geisbert, Thomas W.
    NATURE, 2015, 520 (7549) : 688 - U253
  • [28] Characterization of a serologic marker candidate for development of a live-attenuated DIVA vaccine against porcine reproductive and respiratory syndrome virus
    Vu, Hiep L. X.
    Kwon, Byungjoon
    de Lima, Marcelo
    Pattnaik, Asit K.
    Osorio, Fernando A.
    VACCINE, 2013, 31 (40) : 4330 - 4337
  • [29] Attenuated Salmonella Typhimurium delivery of a novel DNA vaccine induces immune responses and provides protection against duck enteritis virus
    Liu, Xueyan
    Liu, Qing
    Xiao, Kangpeng
    Li, Pei
    Liu, Qiong
    Zhao, Xinxin
    Kong, Qingke
    VETERINARY MICROBIOLOGY, 2016, 186 : 189 - 198
  • [30] Cross-protection against H7N9 influenza strains using a live-attenuated H7N3 virus vaccine
    Carter, Donald M.
    Bloom, Chalise E.
    Kirchenbaum, Greg A.
    Tsvetnitsky, Vadim
    Isakova-Sivak, Irina
    Rudenko, Larisa
    Ross, Ted M.
    VACCINE, 2015, 33 (01) : 108 - 116
12345