Determination of carbamazepine in parenteral nanoemulsions: Development and validation of an HPLC method

被引:15
作者
Kelmann, R. G.
Kuminek, G.
Teixeira, H. F.
Koester, L. S.
机构
[1] Univ Fed Santa Catarina, Dept Ciencias Farmaceut, Ctr Ciencias Saude, BR-88040900 Florianopolis, SC, Brazil
[2] Univ Fed Rio Grande do Sul, Fac Farm, BR-90610000 Porto Alegre, RS, Brazil
关键词
column liquid chromatography; validation; parenteral nanoemulsions; carbamazepine;
D O I
10.1365/s10337-007-0314-7
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
A high performance liquid chromatographic method was developed and validated for the quantitative determination of carbamazepine in intravenous nanoemulsions. The method validation yielded good results with respect to linearity, specificity, precision and accuracy. The method was carried out on a RP-18 column with a mobile phase composed of methanol-water (70:30 v/v) subjected to a gradient of acetonitrile after drug elution, and detection at 286 nm. The linearity in the range of 10.0-50.0 mu g mL(-1) presented a determination coefficient (r(2)) of 0.9996, calculated by least-squares regression; the RSD values for intra-day and inter-day precision for % recovered were < 0.44 and < 1.21%, respectively; and the recovery of corbamazepine from the sample matrix ranged from 94.3 to 104.9%.
引用
收藏
页码:427 / 430
页数:4
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