Non-target-site resistance to PDS-inhibiting herbicides in a wild radish (Raphanus raphanistrum) population

被引：22

作者：

Lu, Huan ^{[1
]}

Yu, Qin ^{[1
]}

Han, Heping ^{[1
]}

Owen, Mechelle J. ^{[1
]}

Powles, Stephen B. ^{[1
]}

机构：

[1] Univ Western Australia, Sch Agr & Environm, AHRI, Crawley, WA 6009, Australia

来源：

PEST MANAGEMENT SCIENCE | 2020年 / 76卷 / 06期

关键词：

wild radish; diflufenican; PDS gene; malathion; cross-resistance; PHYTOENE DESATURASE; METABOLISM; DIFLUFENICAN; EVOLUTION; SOIL; MECHANISMS; BIOTYPE; GENES; 2,4-D; CROP;

D O I：

10.1002/ps.5733

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

BACKGROUND Diflufenican resistance has been reported in wild radish populations since 1998, but the resistance mechanisms have not been investigated. Recently, we identified a wild radish population (H2/10) from the Western Australian grain belt that is resistant (R) to the phytoene desaturase (PDS)-inhibiting herbicide diflufenican. RESULTS Dose-response results showed this R population is 4.9-fold more resistant than the susceptible (S) population based on the LD50 R/S ratio. In addition, the R population also exhibits cross-resistance to the PDS-inhibiting herbicide fluridone. The cytochrome P450 inhibitor malathion reversed diflufenican resistance and partially reversed fluridone resistance in the R population. The full coding sequences of the PDS gene were cloned from the S and R plants and there are natural variations in the PDS gene transcripts/alleles with no correlation to resistance. In addition, the R plants had a level of PDS gene expression that is not significantly different from the S plants. CONCLUSION These results demonstrated that diflufenican resistance in this R wild radish population is likely due to non-target-site based enhanced herbicide metabolism involving cytochrome P450s. (c) 2019 Society of Chemical Industry

引用

页码：2015 / 2020

页数：6

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