Surface reactivity and in vitro toxicity on human bronchial epithelial cells (BEAS-2B) of nanomaterials intermediates of the production of titania-based composites

被引：10

作者：

Vergaro, Viviana ^{[1
,2
]}

Aldieri, Elisabetta ^{[3
,6
]}

Fenoglio, Ivana ^{[4
,5
,6
]}

Marucco, Arianna ^{[4
,5
,6
]}

Carlucci, Claudia ^{[7
]}

Ciccarella, Giuseppe ^{[1
,2
]}

机构：

[1] Univ Salento, Biol & Environm Sci & Technol Dept, Via Monteroni, I-73100 Lecce, Italy

[2] Univ Salento, Ist Nanotecnol, CNR NANOTEC, Via Monteroni, I-73100 Lecce, Italy

[3] Univ Turin, Dept Oncol, Turin, Italy

[4] Univ Turin, Dept Chem, Via P Giuria 7, I-10125 Turin, Italy

[5] Univ Turin, NIS, Turin, Italy

[6] Univ Turin, G Scansetti Interdept Ctr Studies Asbestos & Othe, Turin, Italy

[7] Univ Bari, Dept Chem, Via Orabona 4, I-70126 Bari, Italy

来源：

TOXICOLOGY IN VITRO | 2016年 / 34卷

关键词：

TiO2; Cytotoxicity; Oxidative stress; Inflammation; Lung; Hazard; DIOXIDE NANOPARTICLES; OXIDATIVE STRESS; TIO2; CYTOTOXICITY; DAMAGE; GENOTOXICITY; PARTICLES; FIBROBLASTS; SUNSCREENS; APOPTOSIS;

D O I：

10.1016/j.tiv.2016.04.003

中图分类号：

R99 [毒物学（毒理学）];

学科分类号：

100405 ;

摘要：

Titanium dioxide (TiO2) nanoparticles (NPs) are manufactured worldwide in large quantities for use in a wide range of applications. Evaluating the hazards associated with TiO2 NPs is crucial as it enables risk assessment related to human and environmental exposure. In this study the in vitro human toxicity of a set of TiO2 NPs modified with acetic, oleic and boric acids were studied in order to assess the hazard in view of a future scale-up of the synthesis. The surface reactivity of the powders under simulated solar illumination and in the dark has been evaluated by means of EPR spectroscopy. Human bronchial epithelial cells (BEAS-2B) have been chosen as a model for lung epithelium. Cytotoxicity has been assessed by measuring the cells membrane integrity by lactate dehydrogenase (LDH) assay, and the inflammatory response evaluated as nitric oxide (NO) and TNF-alpha production, and oxidative stress measured as intracellular reduced glutathione (GSH) levels, and induced lipoperoxidation. Aeroxide P25 was used for comparison. The results demonstrated a low photoreactivity and toxic effects lower than Aeroxide P25 of the nano-TiO2 powders, probably as a consequence of the presence of acidic moieties at the surface. (C) 2016 Elsevier Ltd. All rights reserved.

引用

页码：171 / 178

页数：8

共 61 条

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