Discrimination of crude and processed rhubarb products using a chemometric approach based on ultra fast liquid chromatography with ion trap/time-of-flight mass spectrometry

被引：18

作者：

Wang, Min ^{[1
]}

Fu, Jinfeng ^{[1
]}

Guo, Huimin ^{[1
]}

Tian, Yuan ^{[1
]}

Xu, Fengguo ^{[1
,2
]}

Song, Rui ^{[1
,2
]}

Zhang, Zunjian ^{[1
,2
]}

机构：

[1] China Pharmaceut Univ, Key Lab Drug Qual Control & Pharmacovigilance, Minist Educ, Nanjing, Jiangsu, Peoples R China

[2] China Pharmaceut Univ, State Key Lab Nat Med, Nanjing, Jiangsu, Peoples R China

来源：

JOURNAL OF SEPARATION SCIENCE | 2015年 / 38卷 / 03期

关键词：

Chemical markers; Chemometrics; Mass spectrometry; Processing; Rhubarb; DAY REPRODUCIBILITY; PLANT METABOLOMICS; PHENOLIC-COMPOUNDS; TOF-MS; CONSTITUENTS; COMPONENTS; STRATEGY; ARRAY;

D O I：

10.1002/jssc.201401044

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Crude rhubarb subjected to different processing procedures will produce different therapeutic effects that are possibly due to processing-induced variation in chemical composition. In this study, a chemometric approach based on ultra fast liquid chromatography with ion trap/time-of-flight mass spectrometry was established to systematically investigate the chemical variations of rhubarb induced by different processing methods. The approach was validated based on pooled quality-control samples from two perspectives: the individual properties of variables and the bulk properties of samples. Orthogonal partial least squares discriminant analysis was introduced to compare the differences between crude and processed rhubarb products. A total of 20 significantly different markers were screened out and unambiguously/tentatively characterized. This research proved that a chemometric method based on ultra fast liquid chromatography with ion trap/time-of-flight mass spectrometry can comprehensively analyze the chemical variation of herbal medicine and provide evidence for a deeper understanding of the pharmacological activities of processed rhubarb products.

引用

页码：395 / 401

页数：7

共 32 条

[1] Plasma anti-Mullerian hormone as a biomarker for bovine granulosa-theca cell tumors: Comparison with immunoreactive inhibin and ovarian steroid concentrations [J].