Effect of chlorogenic acid on hydroxyl radical

被引：129
|
作者：
Zang, LY
Cosma, G
Gardner, H
Castranova, V
Vallyathan, V
机构：
[1] NIOSH, Exposure Assessment Branch, Hlth Effects Lab Div, Morgantown, WV 26505 USA
[2] NIOSH, Pathol & Phsyiol Res Branch, Hlth Effects Lab Div, Morgantown, WV 26505 USA
[3] Colorado State Univ, Ft Collins, CO 80523 USA
来源：
MOLECULAR AND CELLULAR BIOCHEMISTRY | 2003年 / 247卷 / 1-2期
关键词：
chlorogenic acid; hydroxyl radical; ESR; spin trapping;
D O I：
10.1023/A:1024103428348
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
Chlorogenic acid (CGA) is considered to act as an antioxidant. However, the inhibitory effects of CGA on specific radical species are not well understood. Electron spin resonance (ESR) in combination with spin trapping techniques was utilized to detect free radicals. 5,5-Dimethyl-1-pyrroline-N-oxide ( DMPO) was used as a spin trapping reagent while the Fenton reaction was used as a source of hydroxyl radical (.OH). We found that CGA scavenges .OH in a dose-dependent manner. The kinetic parameters, IC50 and V-max, for CGA scavenging of .OH were 110 and 1.27 muM/sec, respectively. The rate constant for the scavenging of .OH by CGA was 7.73 x 10(9) M-1 sec(-1). Our studies suggest that the antioxidant properties of CGA may involve a direct scavenging effect of CGA on .OH.
引用
收藏
页码：205 / 210
页数：6
相关论文
共 50 条
  • [1] Effect of chlorogenic acid on hydroxyl radical
    Lun-Yi Zhang
    Greg Cosma
    Henry Gardner
    Val Vallyathan
    Vince Castranova
    Molecular and Cellular Biochemistry, 2003, 247 : 205 - 210
  • [2] Effect of Chlorogenic Acid on the Physicochemical and Functional Properties of Coregonus Peled Myofibrillar Protein through Hydroxyl Radical Oxidation
    Guo, Xin
    Qiu, Hengheng
    Deng, Xiaorong
    Mao, Xiaoying
    Guo, Xiaobing
    Xu, Chengjian
    Zhang, Jian
    MOLECULES, 2019, 24 (17):
  • [3] Peroxynitrous acid - Where is the hydroxyl radical?
    Kissner, R
    Nauser, T
    Kurz, C
    Koppenol, WH
    IUBMB LIFE, 2003, 55 (10-11) : 567 - 572
  • [4] OXIDATION OF DMSO AND METHANESULFINIC ACID BY THE HYDROXYL RADICAL
    SCADUTO, RC
    FREE RADICAL BIOLOGY AND MEDICINE, 1995, 18 (02) : 271 - 277
  • [5] Hydroxyl radical mediated degradation of phenylarsonic acid
    Xu, Tielian
    Kamat, Prashant V.
    Joshi, Sachin
    Mebel, Alexander M.
    Cai, Yong
    O'Shea, Kevin E.
    JOURNAL OF PHYSICAL CHEMISTRY A, 2007, 111 (32): : 7819 - 7824
  • [6] Damage of Deoxyribonucleic Acid Caused by Hydroxyl Radical
    Wang Xiao-Xing
    Fang Yan-Fen
    Yang Jing
    Chen Deng-Xia
    Huang Ying-Ping
    Zhang Ai-Qing
    CHINESE JOURNAL OF ANALYTICAL CHEMISTRY, 2011, 39 (05) : 640 - 644
  • [7] EFFECT OF CHLOROGENIC ACID ON SERUM COMPLEMENT
    KOETHE, SM
    BECKER, CG
    FASEB JOURNAL, 1992, 6 (04): : A1453 - A1453
  • [8] Radical scavenging reactions of chlorogenic acid: A pulse radiolysis study
    H. S. Mahal
    T. Mukherjee
    Research on Chemical Intermediates, 2006, 32 : 671 - 682
  • [9] Radical scavenging reactions of chlorogenic acid: a pulse radiolysis study
    Mahal, H. S.
    Mukherjee, T.
    RESEARCH ON CHEMICAL INTERMEDIATES, 2006, 32 (07) : 671 - 682
  • [10] Studies on the antioxidant activity of Lippia citriodora infusion:: Scavenging effect on superoxide radical, hydroxyl radical and hypochlorous acid
    Valentao, P
    Fernandes, E
    Carvalho, F
    Andrade, PB
    Seabra, RM
    Bastos, MD
    BIOLOGICAL & PHARMACEUTICAL BULLETIN, 2002, 25 (10) : 1324 - 1327
12345