In vitro release and anti-herpetic activity of Cymbopogon citratus volatile oil-loaded nanogel

被引：31
作者：
Almeida, Kessiane B. [1 ,2 ]
Araujo, Juliana L. [1 ]
Cavalcanti, Jessica F. [3 ]
Romanos, Maria Teresa V. [3 ]
Mourao, Samanta C. [1 ,2 ]
Amaral, Ana Claudia F. [4 ]
Falcao, Deborah Q. [1 ,2 ]
机构：
[1] Univ Fed Fluminense, Fac Farm, Dept Tecnol Farmaceut, Niteroi, RJ, Brazil
[2] Univ Fed Fluminense, Programa Posgrad Ciencias Aplicadas Prod Saude, Niteroi, RJ, Brazil
[3] Univ Fed Rio de Janeiro, Ctr Ciencias Saude, Inst Microbiol Paulo Goes Ctr Ciencias Saude, Dept Virol, Rio De Janeiro, Brazil
[4] Fiocruz MS, FarManguinhos, Dept Prod Nat, Rio De Janeiro, RJ, Brazil
来源：
REVISTA BRASILEIRA DE FARMACOGNOSIA-BRAZILIAN JOURNAL OF PHARMACOGNOSY | 2018年 / 28卷 / 04期
关键词：
Nanotechnology; Polymeric nanoparticles; Volatile oil; Lemongrass; Hydrogel; Herpes simplex virus; HERPES-SIMPLEX-VIRUS; ANTIBACTERIAL ACTIVITY; POLYMERIC NANOPARTICLES; PLGA NANOPARTICLES; TOPICAL DELIVERY; CELLULAR UPTAKE; DRUG; TYPE-2; NANOCAPSULES; STABILITY;
D O I：
10.1016/j.bjp.2018.05.007
中图分类号：
R914 [药物化学];
学科分类号：
100701 ;
摘要：
This study aimed to prepare hydrogel containing Cymbopogon citratus (DC.) Stapf, Poaceae, volatile oil encapsulated in poly (D,L-lactide-co-glycolide) nanoparticles and to evaluate its in vitro anti-herpetic activity. Polymeric nanoparticles were prepared by solvent emulsification-diffusion method and incorporated in carbomer hydrogels. In vitro release profiles for the nanogel, loaded nanoparticles and hydrogel containing free oil were evaluated by dialysis. Inhibitory activities against Herpes simplex for the formulations were investigated in Vero cells. Hydrogel was developed using nanoparticles with mean diameter of 217.1 nm and negative Zeta potential (-20.5 mV). Volatile oil release profile showed a biphasic pattern with an initial faster release and subsequent sustained phase in all formulations. Nanogel strongly inhibited virus in a non-cytotoxic concentration, 42.16 times lower than free oil, 8.76 and 2.23 times than loaded nanoparticles and hydrogel containing free oil, respectively. These results highlight the potential of nanogel to protect oil against volatilization, control release and improve its anti-herpetic activity. (C) 2018 Sociedade Brasileira de Farmacognosia. Published by Elsevier Editora Ltda.
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收藏
页码：495 / 502
页数：8
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