Analysis of the 5' cDNA sequence of the boar proacrosin binding protein

cDNA sequence of the amino terminal boar proacrosin binding protein was determined by the 5'-end amplification of the boar proacrosin binding protein cDNA employing 5'-RACE PCR and the sequencing of the amplified products by the chain termination method. 5'-RACE PCR products were amplified from total boar testicular RNA using the specific primer sets which are designed from the previously analyzed partial cDNA sequence of the boar proacrosin binding protein. When the amplified products were analyzed by agarose gel electrophoresis, three prominent bands were shown. The amplified products were then cloned into pGEM T-easy vector and were sequenced by base-specific termination method with T7 polymerase. The partial amino terminal cDNA sequence of the boar proacrosin binding protein showed a high homology with that of the previously reported boar testicular protein, Sp32, upon NCBI blast homology search.