The Protective Effect of Cordycepin On Alcohol-Induced Osteonecrosis of the Femoral Head

被引:23
作者
Chen, Yi-Xuan [1 ]
Zhu, Dao-Yu [1 ]
Xu, Zheng-Liang [1 ]
Yin, Jun-Hui [2 ]
Yu, Xiao-Wei [1 ]
Mei, Jiong [1 ]
Gao, You-Shui [1 ]
Zhang, Chang-Qing [1 ,2 ]
机构
[1] Shanghai Jiao Tong Univ, Affiliated Peoples Hosp 6, Dept Orthoped Surg, 600 Yishan Rd, Shanghai, Peoples R China
[2] Shanghai Jiao Tong Univ, Affiliated Peoples Hosp 6, Inst Microsurg Extrem, Shanghai, Peoples R China
基金
中国国家自然科学基金;
关键词
Cordycepin; Osteonecrosis of the femoral head; Alcohol; Osteogenesis; Wnt/beta-catenin pathway; Human bone mesenchymal stem cells; STEROID-INDUCED OSTEONECROSIS; MESENCHYMAL STROMAL CELLS; RAT MODEL; OXIDATIVE STRESS; BONE; ETHANOL; DIFFERENTIATION; INHIBITION; EXPRESSION; MILITARIS;
D O I
10.1159/000480181
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Background: Alcohol abuse is known to be a leading risk factor for atraumatic osteonecrosis of the femoral head (ONFH), in which the suppression of osteogenesis plays a critical role. Cordycepin benefits bone metabolism; however, there has been no study to determine its effect on osteonecrosis. Methods: Human bone mesenchymal stem cells (hBMSCs) were identified by multi-lineage differentiation. Alkaline phosphatase (ALP) activity, RT-PCR, western blots, immunofluorescent assay and Alizarin red staining of BMSCs were evaluated. A rat model of alcohol-induced ONFH was established to investigate the protective role of cordycepin against ethanol. Hematoxylin & eosin (H&E) staining and micro-computerized tomography (micro-CT) were performed to observe ONFH. Apoptosis was assessed by TdT-mediated dUTP nick end labeling (TUNEL). Immunohistochemical staining was carried out to detect OCN and [(]LL Results: Ethanol significantly, suppressed ALP activity, decreased gene expression of OCN and BMP2, lowered levels of RUNX2 protein, and reduced immunofluorescence staining of OCN and COL1 and calcium formation of hBMSCs. However, these inhibitory effects were attenuated by cordycepin co-treatment at concentrations of 1 and 10 mu g/mL. Moreover, it was revealed that the osteo-protective effect of cordycepin was associated with modulation of the Wnt/beta-catenin pathway. In vivo, by micro-CT, TUNEL and immunohistochemical staining of OCN and COL1 we found that cordycepin administration prevented alcohol-induced ONFH. Conclusion: Cordycepin treatment to enhance osteogenesis may be considered a potential therapeutic approach to prevent the development of alcohol-induced ONFH. (C) 2017 The Author(s) Published by S. Karger AG, Basel
引用
收藏
页码:2391 / 2403
页数:13
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