First natural co-occurrence of tomato leaf curl New Delhi virus DNA-A and chili leaf curl betasatellite on tomato plants (Solanum lycopersicum L.) in India

被引：9

作者：

Agnihotri, Aniruddha Kumar ^{[1
]}

Mishra, Sudhakar Prasad ^{[1
]}

Tripathi, Ramesh Chandra ^{[2
]}

Ansar, Mohammad ^{[3
]}

Srivastava, Ashish ^{[4
,5
]}

Tripathi, Indra Prasad ^{[2
]}

机构：

[1] Mahatma Gandhi Chitrakoot Gramoday Vishwavidyalay, Fac Agr, Dept Crop Sci, Satna 485334, Madhya Pradesh, India

[2] Mahatma Gandhi Chitrakoot Gramoday Vishwavidyalay, Fac Sci & Environm, Dept Biol Sci, Satna 485334, Madhya Pradesh, India

[3] Bihar Agr Univ, Dept Plant Pathol, Bhagalpur 813210, Bihar, India

[4] Amity Univ, Amity Inst Virol & Immunol, Sect 125, Noida 201313, India

[5] Univ Edinburgh, Inst Mol Plant Sci, Edinburgh EH9 3BF, Midlothian, Scotland

来源：

JOURNAL OF GENERAL PLANT PATHOLOGY | 2018年 / 84卷 / 06期

关键词：

Chili leaf curl betasatellite; PCR; Rolling circle amplification; Solanum lycopersicum; Tomato leaf curl New Delhi virus; PCR-MEDIATED AMPLIFICATION; INFECTING TOMATO; BIPARTITE BEGOMOVIRUS; UNIVERSAL PRIMERS; GEMINIVIRUSES; BETA; DIVERSITY;

D O I：

10.1007/s10327-018-0807-2

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Tomato leaf curl disease (ToLCD) affected 25% of the tomato crop in Chitrakoot, India and symptomatic leaves were collected for molecular assay. The complete sequences of bipartite begomovirus DNA-A and a betasatellite DNA were amplified. In a sequence analysis, begomovirus DNA-A and betasatellite shared highest sequence identity (91-99%) with Tomato leaf curl New Delhi virus (ToLCNDV) DNA-A and chili leaf curl betasatellite (ChLCB), respectively. The virus was transmitted by whitefly to tomato plants and caused ToLCD symptoms with 70% transmission rate. To our knowledge, this is the first report of the natural occurrence of ToLCNDV and ChLCB in India.

引用

页码：414 / 417

页数：4

共 19 条

[1]

Akhter A, 2014, INT J AGRIC BIOL, V16, P1225

[2] Distinct association of an alphasatellite and a betasatellite with Tomato leaf curl New Delhi virus in field-infected cucurbit [J].

Anwar, Sidra .

JOURNAL OF GENERAL PLANT PATHOLOGY, 2017, 83 (03) :185-188

[3] Subviral agents associated with plant single-stranded DNA viruses [J].

Briddon, RW ;

Stanley, J .

VIROLOGY, 2006, 344 (01) :198-210

[4] Universal primers for the PCR-mediated amplification of DNA β -: A molecule associated with some monopartite begomoviruses [J].

Briddon, RW ;

Bull, SE ;

Mansoor, S ;

Amin, I ;

Markham, PG .

MOLECULAR BIOTECHNOLOGY, 2002, 20 (03) :315-318

[5] Universal primers for the PCR-mediated amplification of DNA 1:: A satellite-like molecule associated with begomovirus-DNA β complexes [J].

Bull, SE ;

Briddon, RW ;

Markham, PG .

MOLECULAR BIOTECHNOLOGY, 2003, 23 (01) :83-86

[6] A New Begomovirus Species Causing Tomato Leaf Curl Disease in Varanasi, India [J].

Chakraborty, S. ;

Pandey, P. K. ;

Banerjee, M. K. ;

Kalloo, G. ;

Fauquet, C. M. .

PLANT DISEASE, 2003, 87 (03)

[7] DETECTION AND DIFFERENTIATION OF WHITEFLY-TRANSMITTED GEMINIVIRUSES IN PLANTS AND VECTOR INSECTS BY THE POLYMERASE CHAIN-REACTION WITH DEGENERATE PRIMERS [J].

DENG, D ;

MCGRATH, PF ;

ROBINSON, DJ ;

HARRISON, BD .

ANNALS OF APPLIED BIOLOGY, 1994, 125 (02) :327-336

[8] Geminiviruses: masters at redirecting and reprogramming plant processes [J].

Hanley-Bowdoin, Linda ;

Bejarano, Eduardo R. ;

Robertson, Dominique ;

Mansoor, Shahid .

NATURE REVIEWS MICROBIOLOGY, 2013, 11 (11) :777-788

[9] Genetic diversity and distribution of a distinct strain of Chili leaf curl virus and associated betasatellite infecting tomato and pepper in Oman [J].

Khan, Akhtar J. ;

Akhtar, Sohail ;

Al-Zaidi, Amal M. ;

Singh, Achuit K. ;

Briddon, Rob W. .

VIRUS RESEARCH, 2013, 177 (01) :87-97

[10] Molecular characterization of a distinct bipartite begomovirus species infecting tomato in India [J].

Kumar, Yogesh ;

Hallan, Vipin ;

Zaidi, Aijaz A. .

VIRUS GENES, 2008, 37 (03) :425-431

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