Functionally Active Gap Junctions between Connexin 43-Positive Mesenchymal Stem Cells and Glioma Cells

被引：5

作者：

Gabashvili, A. N. ^{[1
]}

Baklaushev, V. P. ^{[1
]}

Grinenko, N. F. ^{[2
]}

Levinskii, A. B. ^{[1
]}

Mel'nikov, P. A. ^{[1
]}

Cherepanov, S. A. ^{[1
]}

Chekhonin, V. P. ^{[1
,2
]}

机构：

[1] NI Pirogov Russian Natl Res Med Univ, Medicobiol Fac, Dept Med Nanobiotechnol, Moscow, Russia

[2] VP Serbsky State Res Ctr Forens & Social Psychiat, Dept Fundamental & Appl Neurobiol, Moscow, Russia

来源：

BULLETIN OF EXPERIMENTAL BIOLOGY AND MEDICINE | 2015年 / 159卷 / 01期

基金：

俄罗斯基础研究基金会;

关键词：

mesenchymal stem cells; glioblastoma multiforme; C6; glioma; connexin; 43; gap junctions; NEURAL PRECURSOR CELLS; DIFFERENTIATED GLIOMA; MONOCLONAL-ANTIBODIES; CONDITIONED MEDIUM; PROGENITOR CELLS; TUMOR-CELLS; IN-VITRO; BRAIN; COMMUNICATION; SUPPRESSES;

D O I：

10.1007/s10517-015-2916-7

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

The formation of functional gap junctions between mesenchymal stem cells and cells of low-grade rat glioma C6 cells was studied in in vitro experiments. Immunocytochemical analysis with antibodies to connexin 43 extracellular loop 2 showed that mesenchymal stem cells as well as C6 glioma cells express the main astroglial gap junction protein connexin 43. Analysis of migration activity showed that mesenchymal stem cells actively migrate towards C6 glioma cells. During co-culturing, mesenchymal stem cells and glioma C6 form functionally active gap junctions mediating the transport of cytoplasmic dye from glioma cells to mesenchymal stem cells in the opposite direction. Fluorometry showed that the intensity of transport of low-molecular substances through heterologous gap junctions between mesenchymal stem cells and glioma cells is similar to that through homologous gap junctions between glioma cells. This phenomenon can be used for the development of new methods of cell therapy of high-grade gliomas.

引用

页码：173 / 179

页数：7

共 36 条

[1] Neural stem cells display extensive tropism for pathology in adult brain: Evidence from intracranial gliomas [J].