Differences in structure, physiological stability, electrochemistry, cytotoxicity, DNA and protein binding properties between two Ru(III) complexes

被引:163
作者
Tan, Caiping [1 ]
Liu, Jie [1 ,3 ]
Li, Hong [2 ]
Zheng, Wenjie [3 ]
Shi, Shuo [1 ]
Chen, Lanmei [1 ]
Ji, Liangnian [1 ]
机构
[1] Sun Yat Sen Univ, Sch Chem & Chem Engn, MOE Lab Bioinorgan & Synth Chem, Guangzhou 510275, Peoples R China
[2] S China Normal Univ, Dept Chem, Guangzhou 510631, Peoples R China
[3] Jinan Univ, Dept Chem, Guangzhou 510632, Peoples R China
基金
中国国家自然科学基金; 高等学校博士学科点专项科研基金;
关键词
Ru(III) complexes; anticancer drug; dpq;
D O I
10.1016/j.jinorgbio.2007.09.008
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A novel Ru(III) complex, mer-[RuCl3(CH3CN)(dpq)] (1), has been synthesized and characterized by X-ray diffraction, where dpq = dipyrido[3,2-d:2',3'-f]quinoxaline. Its chemical and biological properties have been intensively compared with those of mer-[RuCl3(DMSO)(dpq)] (DMSO = dimethyl sulfoxide) (2). It has been found that the stability in buffered solutions and the reduction potential for the Ru-III/Ru-II couple can be modulated by changing the small molecule bonded to the Ru(III) center. Interactions of 1 with DNA have been investigated by DNA melting experiments, DNA competitive binding with EB (ethidium bromide), plasmid DNA cleavage experiments and viscosity measurements. The interaction of 1 and 2 with BSA (bovine serum albumin) has also been studied using fluorescent quenching method. The experimental results show that 1 exerts higher affinity towards DNA and BSA than 2 does. The cytotoxicity of 1 has been evaluated by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) method, and 2 shows slightly higher anticancer potency than 1 does against all the cell lines screened. Attempts are made to clarify the possible antitumor mechanisms of these two complexes by analyzing the experimental results presented. (c) 2007 Elsevier Inc. All rights reserved.
引用
收藏
页码:347 / 358
页数:12
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