Analysis of biochemical features of ST8 α-N-acetyl-neuraminide α2,8-sialyltransferase (St8sia) 5 isoforms

被引：3

作者：

Araki, Erino ^{[1
,2
]}

Hane, Masaya ^{[1
,2
,3
]}

Hatanaka, Rina ^{[1
,2
]}

Kimura, Ryota ^{[1
,2
]}

Tsuda, Kana ^{[1
,2
]}

Konishi, Miku ^{[4
]}

Komura, Naoko ^{[4
]}

Ando, Hiromune ^{[4
]}

Kitajima, Ken ^{[1
,2
,3
]}

Sato, Chihiro ^{[1
,2
,3
]}

机构：

[1] Nagoya Univ, Grad Sch Bioagr Sci, Chikusa Ku, Nagoya, Aichi 4648601, Japan

[2] Nagoya Univ, Biosci & Biotechnol Ctr, Chikusa Ku, Nagoya, Aichi 4648601, Japan

[3] Nagoya Univ, Inst Glycocore Res iGCORE, GlycobioMed Res Ctr iGMED, Chikusa Ku, Nagoya, Aichi 4648601, Japan

[4] Gifu Univ, Inst Glycocore Res iGCORE, 1-1 Yanagido, Gifu 5011193, Japan

来源：

GLYCOCONJUGATE JOURNAL | 2022年 / 39卷 / 02期

关键词：

Sialic acid; Sialyltransferase; Melanoma; Ganglioside; isoform; MOLECULAR-CLONING; EXPRESSION; SYNTHASE; GLYCOPROTEINS; RETENTION; ANTIGENS; ACIDS;

D O I：

10.1007/s10719-021-10034-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Gangliosides are important components of the membrane and are involved in many biological activities. St8sia5 is an alpha 2,8-sialyltransferase involved in ganglioside synthesis, and has three isoforms. In this study, we analyzed the features of three isoforms, St8sia5-S, -M, and -L that had not been analyzed, and found that only St8sia5-L was localized in the Golgi, while the majority of St8sia5-M and -S were localized in the ER. The localization of Golgi of St8sia5 depended on the stem region. In addition, the incorporation of exogenous GD3 was upregulated only in St8sia5-L expressing cells. Taken together, the localization of St8sia5 is important for the activity of the enzyme.

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收藏

页码：291 / 302

页数：12

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