Flow-injection chemiluminescence determination of trace calf thymus DNA

被引:0
作者
Zhou, M [1 ]
Ma, YJ [1 ]
Jin, XY [1 ]
Teng, XL [1 ]
Zhang, ZY [1 ]
Chen, H [1 ]
机构
[1] NW Normal Univ, Inst Chem, Lanzhou 730070, Peoples R China
关键词
chemiluminescence; flow-injection; calf thymus DNA; imidazole; rhodamine B;
D O I
暂无
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
A flow injection procedure for the determination of calf thymus DNA (CT DNA) over the range 2.1x10(-6)similar to2.1x10(-1) mug mL(-1) is described, based on measurement of the enhanced chemiluminescence emission of rhodamine B-Ce(W) system, activated by imidazole-HCl buffer solution. This method is highly sensitive, with the linearity range broadened to five orders of magnitude. It has been applied to determine CT DNA in synthetic sample with satisfactory results.
引用
收藏
页码:1051 / 1053
页数:3
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