Structure of phosphorylated and sulfated polysaccharides from lipopolysaccharide of marine bacterium Marinicella litoralis KMM 3900T

被引：6

作者：

Kokoulin, Maxim S. ^{[1
]}

Lizanov, Igor N. ^{[1
,2
]}

Romanenko, Lyudmila A. ^{[1
]}

Chikalovets, Irina, V ^{[1
,2
]}

机构：

[1] Russian Acad Sci, GB Elyakov Pacific Inst Bioorgan Chem, Far Eastern Branch, Prospekt 100 Let Vladivostoku 159, Vladivostok 690022, Russia

[2] Far Eastern Fed Univ, Ul Sukhanova 8, Vladivostok 690950, Russia

来源：

CARBOHYDRATE RESEARCH | 2020年 / 490卷

关键词：

Marine bacterium; Marinicella litoralis; lipopolysaccharide; Polysaccharide; Methyl phosphate; 3-O-Methyl-D-mannose; CARBOHYDRATE; HYDROLYSIS;

D O I：

10.1016/j.carres.2020.107961

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Two polysaccharide fractions were obtained by mild acid degradation of the lipopolysaccharide of the marine bacterium Marinicella litoralis KMM 3900(T). The major polysaccharide was found to contain glycerol 1-phosphate (PGro) and methyl phosphate substituents (PMe), and the following structure of its disaccharide repeating unit was established by sugar analysis, dephosphorylation, Smith degradation, and 1D and 2D NMR spectroscopy: -> 4)-alpha-L-Rhap2PGro(similar to 40%) (1 -> 3)-beta-D-Manp6PMe(similar to 80%)-(1 -> . The minor polysaccharide was shown to consist of 4-O-sulfate-D-mannopyranosyl residues, non-stoichiometric methylated at O-3 and acetylated at O-6: -> 2)-alpha-D-Manp3R4S6Ac(similar to 75%)-(1 ->, where R is Me (85%) or H (15%).

引用

页数：7

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