Purification, structural characterization and immunomodulatory activities of a polysaccharide from the fruiting body of Morchella sextelata

被引：57
作者：
Kuang, Meng -Ting [1 ]
Xu, Jing-Yue [1 ]
Li, Jin-Yu [1 ]
Yang, Liu [1 ]
Hou, Bo [1 ]
Zhao, Qi [2 ]
Hu, Jiang- Miao [1 ]
机构：
[1] Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochemistry & Plant Resources Wes, Yunnan Key Lab Nat Med Chem, Kunming 650201, Peoples R China
[2] Chinese Acad Sci, Kunming Inst Bot, Key Lab Plant Divers & Biogeog East Asia, Kunming 650201, Peoples R China
来源：
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 2022年 / 213卷
关键词：
Morchella sextelata polysaccharide; Structural characterization; Immunomodulatory activities; NEUTRAL POLYSACCHARIDE; ANTIOXIDANT; ANTITUMOR; SECRETION; MUSHROOM;
D O I：
10.1016/j.ijbiomac.2022.05.096
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A novel polysaccharide (MSP-1) was isolated from the fruiting body of Morchella sextelata and purified using DEAE-52 and Sephadex G-75. The molecular weight of MSP-1 was 1.17 x 10(4) Da, as detected by HPLC analysis. The monosaccharide composition of MSP-1 was mannose and glucose at a ratio of 1.00: 1.25. Methylation and NMR results revealed that the backbone of MSP-1 was composed of & RARR;4)-beta-D-Manp-(1 ->, -> 4)-beta-D-Glcp-(1 ->, -> 4)-alpha-D-Glcp-(1 ->, and -> 4, 6)-alpha-D-Glcp-(1 ->. SEM images of MSP-1 presented a dense network structure with porous characterizations. The immunomodulatory activities of MSP-1 were evaluated using RAW264.7 cells, and the results showed that MSP-1 promoted proliferative and phagocytic activity and increased the production of NO, TNF-alpha and IL-6. These results indicated that MSP-1 exhibited significant immunomodulatory activities.
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收藏
页码：394 / 403
页数：10
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