Thickening of Ectatic Cornea through Regeneration Using Decellularized Corneal Matrix Injectable Hydrogel: A Strategic Advancement to Mitigate Corneal Ectasia

被引:4
作者
Chameettachal, Shibu [1 ]
Puranik, Charuta J. [2 ]
Veluthedathu, Mohamed Nijas [1 ]
Chalil, Najathulla Bhagavathi [3 ]
John, Renu [1 ]
Pati, Falguni [1 ]
机构
[1] Indian Inst Technol Hyderabad, Dept Biomed Engn, Hyderabad 502284, Telangana, India
[2] Oculus Regenerus Eye Care & Res Ctr, Hyderabad 500008, Telangana, India
[3] Indian Inst Technol Hyderabad, Dept Mat Sci & Met Engn, Hyderabad 502284, Telangana, India
关键词
corneal ectasia; stromal depletion; corneal thinning; decellularized cornea matrix hydrogel; corneal regeneration; RECOMBINANT HUMAN COLLAGEN; EXTRACELLULAR-MATRIX; QUANTITATIVE-ANALYSIS; CROSS-LINKING; KERATOCONUS; MICROSTRUCTURE; BIOMECHANICS; MICROSCOPY; CELLS;
D O I
10.1021/acsabm.1c00821
中图分类号
TB3 [工程材料学];
学科分类号
0805 ; 080502 ;
摘要
Ectatic corneal diseases are a group of eye disorders characterized by progressive thinning and outward bulging of the cornea, resulting in vision impairment. A few attempts have been made to use cornea-derived extracellular matrix hydrogels for corneal tissue engineering; however, no studies have investigated its application in corneal ectasia. In this study, we have first developed an animal surgical model that mimics a few specific phenotypes of ectatic cornea. Later, we investigated the potential of decellularized cornea matrix hydrogels (dCMH) from both human and bovine sources in increasing the thickness of the cornea in the developed surgical model. Our data advocate that surgical stromal depletion can be followed to establish ectatic models and can also provide information on the biocompatibility of materials, its integration with native stroma, degradation over time, and tissue remodeling. We observed that dCMH from both sources could integrate with ectatic thin corneal stroma and helps in regaining the thickness by regenerating a reasonably functional and transparent stroma; however, no significant difference was spotted between the dCMH made from human and bovine corneal tissue sources. Hence, this study is a promising step toward developing a non-invasive technique for the treatment of corneal ectasia by using dCMH.
引用
收藏
页码:7300 / 7313
页数:14
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